Mycotoxin detection common products vomitoxin test strips, also known as DON test strips, can be used to quickly and accurately quantitatively determine the content of vomitoxin in raw materials or processed products such as grains and feeds.
Because of the vomiting of pigs and the name of vomitoxin, China has made a corresponding limit standard for vomitoxin, and the products that do not meet the limit standard of vomitoxin content are not allowed to be sold to the outside world, so as not to pose a threat to life and health after eating.
The detection of vomitoxin by the test strip needs to be used with the FD-600 D-600 DON detector, which is different from the traditional qualitative detection of colloidal gold, and realizes the upgrading from rough qualitative to accurate quantification, quantitative display, more intuitive detection results, more accurate judgment, and reduces the error caused by human judgment.
How to operate the vomitoxin test strip
1. Take 300-500g of representative samples, crush them for 1 minute, mix the crushed samples and take a second sample
2. Accurately weigh 5 005 g of the crushed sample is placed in a 50 ml centrifuge tube;
3. Add 25ml of sample extraction solution and shake for 3 minutes
4. After shaking extraction, let stand for a few seconds, and take 1ml of liquid in 1Centrifuge in a 5 ml tube, 4,000 rpm for 2 min;
5. Take the centrifugal supernatant and dilute it according to the following ratio: 100 l centrifugal supernatant + 500 l sample diluent;After mixing well with a vortex shaker for 5 seconds, take 100 l of the diluted liquid and add it to the loading well of the test card
6. Place the detection card in a 37°C incubator at constant temperature and incubate with a lid for 8 minutesClick the standard curve on the detector screen, select the standard curve corresponding to the sample, and then insert the printed end of the test card item or the end of the sample filling hole into the detection socket of the instrument facing up and forward, and test and print the result.
Precautions for vomitoxin test strip detection:
1. Different batches of test cards, ID cards and diluents cannot be mixed
2. Do not touch the white film surface of the test card to avoid affecting the accuracy of the test results after contamination;
3. After the test card is incubated for 8 minutes, please test immediately (it is recommended to complete the reading of all test cards within 2 minutes after the end of incubation);
4. Precautions for the use of other products, please refer to the instructions for details