Here's a brief introduction to UV analyzer calibration:
1. Scope
This specification is applicable to the calibration of various ultraviolet analyzers that use the fluorescence emitted by substances under the irradiation of ultraviolet light sources to observe, analyze, and photograph substances.
2. Cited documents
The following documents are referenced in this specification:
JJG 879 Ultraviolet Radiation Illuminance Meter.
GB T 19258 ultraviolet germicidal lamp.
QB T2989 Ultraviolet high-pressure mercury lamp ultraviolet irradiance and electrical parameter measurement method.
Where a reference is dated, only the dated version applies to this specification;The most recent version of an undated referenced document (including all change orders) applies to this specification.
3. Terminology
3.1 Fluorescence.
A photoluminescent phenomenon of cold luminescence. Light produced by a radiative transition between states of the same degree of multiplicity. When the light stops emitting, the luminescence disappears.
4. Overview
The ultraviolet analyzer is an instrument that uses the ultraviolet light source to emit and filter out the ultraviolet radiation after the residual visible light, and uniformly irradiates the fluorescent sample to be analyzed. According to the different methods of observing fluorescence, ultraviolet analyzers are divided into reflection type and transmission type. The commonly used wavelengths of the reflectance ultraviolet analyzer are 254nm and 365nm, which is suitable for fluorescence detection and analysis of various opaque materialsTransmission UV analyzers commonly use radiation in 280 nm and 320 nm, which is suitable for the observation and shooting of transparent samples such as DNA and RNA electrophoresis gels. The ultraviolet analyzer is composed of an ultraviolet light source, a filter, and an operating table. Figure 1 is a structural diagram of the UV analyzer.
5. Metrological characteristics
The metrological characteristics of the ultraviolet analyzer include: peak wavelength indication error, ultraviolet irradiance, and various indicators are shown in Table 1
6. Calibration conditions
6.1 Environmental conditions.
Calibrated room temperature is (23°5), relative humidity is 85%, the instrument should be placed in a dark room or shading for use, and there is no strong vibration source nearby.
6.2. Calibration equipment and supporting equipment.
The operating wavelength range of the spectrometer is 250380 nm, and the maximum allowable error of the wavelength indication is 10 nm。
6.2.2. Ultraviolet irradiance meter.
The performance index of the ultraviolet irradiance meter refers to the requirements above the secondary ultraviolet irradiance meter in JJG879;The uncertainty of ultraviolet irradiance expansion is not greater than urel=15%(k=2).
6.2.3 rulers.
Minimum indexing value: 1mm.
That's itUV analyzer calibrationrelated content.