The researchers successfully utilized cyanobacterial biomass as the tip pA new ingredient for the pastoris platform. The extended toolkit shows promising results in providing a sustainable alternative for enzyme expression. **Dr. Doris Schieder, Head of the Department of Bioresource Chemistry, Straubing Campus for Biotechnology and Sustainability, Technical University of Munich, Germany, has a rising demand for industrial enzymes due to the growing need to shift to more sustainable industrial processes. Our study outlines a new enzyme production method that utilizes pUntapped potential of cyanobacterial biomass within the Pastoris platform.
Highlighting the nature of the study, panel leader Dr Schieder said: "Our work reveals the potential of cyanobacteria biorefineries to support enzyme production.
This achievement stems from a broad, multidisciplinary approach. We characterized and expanded a combinatorial library that simplified pPastoris works to improve efficiency. Rigorous selection of these libraries resulted in potent enzyme-producing strains, which enabled the development of an efficient feed-batch strategy for Appa E. coli phytase expression.
A key factor is the successful utilization of Nostoc SP. DE1 biomass hydrolysate is a substrate for fermentation and is a viable and sustainable alternative to traditional feedstocks. Korbinian Sinzinger underscored the importance of the study, saying: "Our findings suggest that the extended PNot only does the Pastoris Toolkit produce high-performance producer strains, but it also provides a greener pathway for enzyme expression, meeting a critical need for sustainable bio-based production.
This ** was published in the Journal of Biological Resources and Bioproducts.
More information:Korbinian Sinzinger et al, Pichia Enzyme Production Platform: From Combinatorial Library Screening to Benchtop Fermentation of Residual Cyanobacterial Biomass, Journal of Bioresources and Bioproducts (2023). doi： 10.1016/j.jobab.2023.12.005