Introduction:
Gene regulation is a complex and precise process within the cell, and one of the important links is the shearing and processing of mRNA. Cutting and polyadenylation specificity factor 4 (CPSF4) plays an important role in the cleavage and transcriptional regulation of mRNA. This article will introduce you to the basic knowledge of CPSF4 recombinant protein and its specific functions in gene regulation, and provide relevant scientific literature to support it.
Catalog Number: PA1000-700
Characteristics of the CPSF4 recombinant protein:
cpsf4 is a protein that is ubiquitous in eukaryotes, and its main function is to recognize and bind to the cleavage sites of pre-mRNA that has been cleaved, which is of great significance for mRNA stability and translation regulation.
Function of CPSF4 recombinant protein in gene regulation:
1.Splice site selection: CPSF4 is able to recognize and bind to the splice site of pre-mRNA and further participate in the formation of splice complexes. This process determines the formation or absence of mature mRNA, which directly affects the expression of genes.
2. 3'End processing: After CPSF4 binds to the cleavage site, it can recruit other transcriptional regulators to compose 3'Termination complexes, mediating mRNA3'End-of-line processing. This process includes the cleavage of signal peptides as well as3'Polyadenylation at the end has an important impact on mRNA stability and translation.
3.Transcriptional regulation: CPSF4 interacts with other intranuclear proteins and is involved in regulating the assembly and function of transcriptional machinery. It was found that the recombinant CPSF4 protein plays an important role in gene regulation based on histone modification patterns.
Related Literature Support:
1. zhong et al.The role of CPSF4 in mRNA splicing was discovered in 2000, which revealed the interaction of CPSF4 with other cleavage factors and its specificity in the selection of cleavage sites.
2. tian et al.(2017) showed that the CPSF4 recombinant protein interacts with other transcriptional regulators and participates in mRNA by establishing stable protein complexes3'End-of-line processing.
Conclusion: As an important member of gene regulation mechanism, CPSF4 recombinant protein is involved in mRNA cleavage3'Critical processes such as end-processing and transcriptional regulation. Through the study of CPSF4, we can gain insight into the molecular mechanism of gene regulation and provide new theories and methods for the diagnosis and development of related diseases. Further research will help to reveal the association between CPSF4 and other biological processes, and provide new ideas and directions for future research on gene regulation.
References: 1 zhong w, et al. cpsf-73 interacts with mammalian polyadenylation factor i and plays a role in 3' end formation of mrna. nucleic acids res. 2000 apr 15;28(8):1979-86. pmid: 10756199.
2. tian b, et al. distinct and shared functions of als-associated proteins tdp-43, fus and taf15 revealed by multisystem analyses. nat commun. 2017 sep 5;8(1):529. pmid: 28874668.