An optical microscope is a common experimental instrument used to observe microscopic structures. In order to ensure the accuracy and reliability of the microscope, the microscope needs to be calibrated before making observations. This article will describe the protocols and procedures for the metrological calibration of optical microscopy.
1. The purpose of microscope calibration
The calibration of the optical microscope is to ensure the accuracy and accuracy of the microscope and to obtain reliable data when observing and measuring the microstructure. With calibration, errors in the microscope itself can be eliminated and the precision and accuracy of observation can be improved.
Second, the steps of microscope calibration
Adjust the light source: First, place the microscope under the light source and adjust the brightness and uniformity of the light source so that the viewing area has enough light and appropriate contrast.
Adjust the scope: Next, adjust the focal length of the scope so that the field of view of the eyepiece is clear and distortion-free. This can be achieved by turning the focus wheel of the eyepiece.
Adjust the objective: Then, select the appropriate objective and adjust the focal length of the objective so that the image of the observed sample is clearly visible. Again, this can be achieved by turning the focus wheel of the nosepiece.
Calibration scale: In the field of view of the microscope, there is usually a scale that measures the length or diameter of the sample. The step to calibrate the scale is to measure it under the microscope with a standard sample of known length, and then adjust the scale of the microscope to match the standard length.
Calibrating the eyepiece micrometer: The eyepiece micrometer of a microscope is typically used to measure the length or diameter of a sample. The procedure for calibrating the eyepiece microscope is to measure under a microscope using a standard sample of known length, and then adjust the scale of the eyepiece micrometer to match the standard length.
Check focus: After Zui, check the microscope's focusing system to ensure that clear images are obtained under different viewing conditions.
3. Precautions
When calibrating the microscope, care should be taken to keep the microscope clean and tidy to avoid dust or other contaminants affecting the observations.
When calibrating the scale and eyepiece micrometer, a standard sample of known length should be used to ensure accurate measurements.
When performing calibration, follow the microscope's instruction manual or relevant standard operating procedures to ensure correct and safe operation.
During calibration, the various parts of the microscope should be carefully observed to ensure that they are not damaged or malfunctioning, so as not to affect the accuracy of the observations.
In conclusion, optical microscope calibration is an important step to ensure that microscope observations are accurate and reliable. The main steps of calibration include adjusting the light source, eyepiece, and objective lens, calibrating the scale and eyepiece micrometer, and checking the focusing system. During calibration, care should be taken to keep the microscope clean and tidy, calibrate with standard samples, and follow operating procedures. With proper calibration, the precision and accuracy of microscope observation can be improved, providing a reliable data basis for scientific research and experiments.