Oyster mushroom, as a common edible mushroom, is rich in nutritional value and delicious in taste, and is deeply loved by consumers. The large-scale production of oyster mushrooms is inseparable from the cultivation of mushrooms. Let's take an in-depth look at the cultivation process of oyster mushroom species.
The first thing we need to understand is the material preparation stage. At this stage, we need to prepare the mother species of Oyster mushroom, also known as the first-order species. The mother seed is the basis of the culture, and its quality directly affects the growth and yield of subsequent strains. At the same time, we also need to prepare the medium for cultivating secondary and ** species. The culture medium is the nutrient for the growth of the fungus**, which is usually made from wheat grains, corn flour, soybean meal, bran, etc. These substances are rich in nutrients such as proteins, sugars, minerals and vitamins, which can meet the needs of the mycelium growth of oyster mushrooms.
Next, we move on to the disinfection process. At this stage, all materials and utensils need to be strictly disinfected to prevent contamination by miscellaneous bacteria. The presence of miscellaneous fungi will compete with the oyster mushroom species for nutrition and living space, which will seriously affect the growth and yield of the mushroom. Commonly used disinfection methods include high-temperature steam disinfection, alcohol wiping, ultraviolet irradiation, etc. Among them, high-temperature steam disinfection is the most commonly used and effective method, which can kill most microorganisms by high temperature and high pressure.
Then, we move on to the media making stage. At this stage, we mix the sterilized medium material in a certain proportion, add the appropriate amount of water, stir well, and pour it into the culture flask. We then put the flasks into an autoclave for sterilization. Autoclave sterilization is a very effective sterilization method that kills all surviving microorganisms by steam at high temperature and pressure, ensuring the sterility of the culture medium.
Next, we move on to the inoculation phase. At this stage, we need to wait for the medium to cool down to a suitable temperature before inoculating the mother seed into the medium. Attention should be paid to aseptic operation during inoculation to avoid the introduction of miscellaneous bacteria. In general, we use a needle or a spatula to gently scrape the mother seed into the medium.
Then, we move on to the cultivation phase. At this stage, we incubate the inoculated flasks at the right temperature, humidity and light. The culture temperature of Pleurotus ostreatus species is generally 24-28 and the humidity is 60-70%. In a suitable environment, the mycelium of Pleurotus ostreatus will grow rapidly, forming a rich mycelial network.
Finally, we move on to the observation and transfer phase. At this stage, we need to regularly observe the growth of mycelium, and if we find any bacterial contamination or poor growth, we need to deal with it in time. At the same time, when the mycelium is full of medium, we need to carry out a transfer, that is, transplant a part of the mycelium to a new medium and continue the culture.
The above are the basic culture steps of oyster mushroom species. However, different oyster mushroom varieties and cultivation conditions may vary, so in practice, they need to be adjusted on a case-by-case basis. In addition, culture culture is a highly technical job that requires professional knowledge and skills, as well as patience and care. Only in this way can we cultivate high-quality oyster mushroom strains and lay a solid foundation for the large-scale production of oyster mushrooms.