Streptavidin-modified rare earth europium polystyrene fluorescent microspheresStreptavidin-modified rare earth europium polystyrene fluorescent microspheres are biocompatible fluorescent microspheres with high luminescence performance and good stability. It is obtained from polystyrene microspheres modified with streptavidin, which can be used in biomolecular detection, immunoassays, tracer labeling, and other applications.
The preparation of such fluorescent microspheres consists of two main steps: the synthesis of polystyrene microspheres first, followed by streptavidin modification. In the synthesis of polystyrene microspheres, styrene monomers polymerize in the presence of peroxide initiators to form polystyrene microspheres. To obtain homogeneous beads, the polymerization reaction needs to be performed in a mixture of water and non-polar solvents. Next, in order to perform streptavidin modification, the surface of the beads needs to be activated to make them reactive.
During streptavidin modification, streptavidin is immobilized on the surface of the microspheres, giving it the ability to bind specifically to the biomolecule. Streptavidin is a protein extracted from Streptomyces with high affinity and specific recognition ability. Through interactions with biomolecules, streptavidin can bind fluorescent microspheres to target molecules.
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qyzyl2023.12.12