DBCO-Cy7 and DBCO-Cy5 are two commonly used near-infrared fluorescent dyes that can both be used for bioimaging, but there are also some differences in some aspects.
There are some differences between DBCO-CY7 and DBCO-CY5 in terms of spectral properties, chemical structure and fluorescence properties.
Spectral properties. The excitation wavelength of DBCO-CY7 is 640 nm and the emission wavelength is about 680 nmThe excitation wavelength of DBCO-Cy5 is 640nm and the emission wavelength is about 710nm. Therefore, the fluorescence emission wavelength of DBCO-CY7 is shorter than that of DBCO-CY5 and is more easily covered by the detection range of the spectroscopic instrument.
Chemical structure. The chemical structures of DBCO-CY7 and DBCO-CY5 are slightly different. DBCO, a dibenzocyclooctynyne, is one of the commonly used click chemical reactants. In dbco-cy7, dbco is connected to a seven-membered ring structure, while in dbco-cy5, dbco is connected to a five-membered ring structure. This different chemical structure leads to differences in their fluorescence properties and stability.
Fluorescence performance and stability.
DBCO-Cy7 has a high fluorescence quantum yield of 03, while DBCO-Cy5 has a lower fluorescence quantum yield of 02 or so. As a result, DBCO-Cy7 has a higher fluorescence brightness and is easier to detect. In addition, DBCO-Cy7 has relatively good stability and is not easy to deteriorate during long-term storage and use.
Since both DBCO-CY7 and DBCO-CY5 have excitation and emission wavelengths in the near-infrared region, they can both be used for near-infrared imaging. However, when choosing which dye to use, there is a need to consider the experimental needs and target characteristics.
If higher fluorescence brightness and a wider detection range are required, DBCO-CY7 can be used. If a more stable and non-perishable dye is required, DBCO-Cy7 can be used.