The ultra-micro spectrophotometer is a high-sensitivity optical analysis instrument, which is widely used in biomedicine, chemistry, environmental science and other fields. This article will describe the use of microvolume spectrophotometers, including sample preparation, instrument setup, measurement procedures, and data processing.
1. Sample preparation.
Before using a microvolume spectrophotometer, the sample to be tested needs to be prepared. For biological samples, it is often necessary to dilute the sample appropriately to reduce the concentration and avoid the effects of light scattering. For chemical samples, appropriate treatment and pretreatment are required depending on the specific analytical method.
2. Instrument settings.
Before using a microvolume spectrophotometer, you need to set up the instrument. First, the appropriate measurement wavelength and bandwidth need to be selected, depending on the nature of the sample to be measured and the measurement requirements. Secondly, it is necessary to set up a suitable sample cell and reference cell to eliminate background interference and improve measurement accuracy. Finally, it is also necessary to adjust the light source, monochromator, detector and other components of the instrument to ensure the accuracy and reliability of the measurement results.
3. Measurement steps.
Turn on the power of the ultra-microvolume spectrophotometer and start the software control system.
The sample to be tested and the reference sample were added to the corresponding sample cell and reference cell, respectively.
Adjust the light source, monochromator, detector and other components of the instrument to keep the instrument in the best measurement state.
Enter the corresponding measurement parameters such as wavelength, bandwidth, sample cell, and reference cell in the software control system.
Start the measurement and record the measurement data.
During the measurement process, it is necessary to pay attention to the state of the instrument and the changes in the measurement results. If there is an abnormality, it needs to be dealt with in a timely manner.
After the measurement, turn off the power of the instrument and clean the bench and instrument surfaces.
4. Data processing.
Once the measurement is complete, the measurement data needs to be processed and analyzed. First, the data needs to be calibrated and corrected to eliminate background interference and errors. Secondly, the data needs to be processed and analyzed according to specific analysis methods in order to draw the right conclusions and results. Finally, the data needs to be organized into ** or charts for subsequent reporting and summarization.
In conclusion, the ultra-micro spectrophotometer is a highly sensitive optical analysis instrument with broad application prospects. In the process of use, it is necessary to pay attention to the accuracy of instrument settings and measurement steps to ensure the accuracy and reliability of measurement results. At the same time, the measurement data needs to be processed and analyzed correctly in order to draw the right conclusions and results.