the styryl dye rh414 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons.
RH 421 is a voltage-sensitive styrene-based dye that penetrates lipid bilayers. Rh 421 is a chromogenic substrate of -galactosidase, and due to its special chemical properties, Rh 421 is widely used in biomedical research as a fluorescent probe to monitor potential changes in cell membranes. Under normal physiological conditions, the fluorescence intensity of Rh 421 is weak, but when the cell membrane is depolarized, the fluorescence intensity of Rh 421 is significantly enhanced. This property makes RH 421 an important tool for studying neuronal activity and cardiac electrophysiology.
In the field of neuroscience, RH 421 is used to monitor the electrical activity of neurons. When neurons are stimulated, their membrane potential changes, resulting in enhanced fluorescence intensity of RH 421. This change can be used to understand the process by which neurons transmit information and the excitability of neurons. By observing changes in RH 421 fluorescence, scientists can delve into the functions and mechanisms of the nervous system.
RH 421 also plays an important role in cardiac electrophysiology studies. In the heart, the electrical activity of cardiomyocytes is done by changes in membrane potential. RH 421 can penetrate the lipid bilayer of cardiomyocytes and change the fluorescence intensity as the membrane potential changes. By monitoring changes in RH 421 fluorescence, scientists can understand the electrophysiological properties of cardiomyocytes as well as the rhythm of the heart's electrical activity.
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