This article is the original of the Translational Medicine Network, **Please indicate the source
Author: rainbow
Reading guide:Circular RNA is recognized for its important regulatory function in various cancers. However, its regulatory role in non-small cell lung cancer (NSCLC) remains largely unknown.
On December 5, Gao Wen's team from Huadong Hospital affiliated to Fudan University published a paper entitled "Circular RNA Circatp9A Promotes Non-small Cell Lung Cancer Progression by Interacting with Hur and" in the journal Journal of Experimental & Clinical Cancer Research by promoting extracellular vesicles-mediated macrophage M2 polarization**, the results of the study showed:Circatp9A may be a promising diagnostic marker and target for non-small cell lung cancer (NSCLC).
Background:
Non-small cell lung cancer (NSCLC) is the predominant type of lung cancer, accounting for approximately 85% of all reported lung cancer instances. It is further divided into various subtypes, with adenocarcinoma, squamous cell carcinoma, and large cell carcinoma being the most prevalent.
Circular RNAs (circRNAs) are a unique class of non-coding RNAs characterized by a circular structure with covalent closure, unlike linear RNAs. Dysregulation of circRNA expression is common in various cancer types and is often associated with disease progression and prognosis. Some circRNAs act as oncogenes to promote tumor growth, metastasis, and drug resistance, while others have tumor-suppressing effects. Given the multifaceted role that circRNAs play in cancer biology, they have great potential as innovative cancer diagnostic biomarkers and targets.
The study found
In the study, the researchers elucidated the functional role of CIRCATP9A in non-small cell lung cancer (NSCLC). Researchers have demonstrated through in vivo and in vitro experiments that CircatP9A can promote the progression of NSCLC. Circatp9A can interact with the HUR protein to form RNA-protein complexes, which subsequently enhance the mRNA and protein levels of the target gene NUCKS1. The PI3K AKT MTOR signaling pathway was identified as a downstream pathway of the CircatP9A hur nucks1 axis. HNRNPA2B1 can mediate the incorporation of Circatp9A into extracellular vesicles (EVS). Subsequently, these CircATP9A-containing EVS induce an M2 phenotype in tumor-associated macrophages (TAMs), thereby promoting the development of NSCLC.
Circatp9A promotes non-small cell lung cancer
(NSCLC) cell proliferation, invasion, and migration
To investigate the expression of Circatp9A in non-small cell lung cancer (NSCLC) cell lines, the researchers performed QRT-PCR experiments on four different NSCLC cell lines. The results showed a significant increase in the abundance of CircatP9A in these cell lines compared to Beas-2B cells, and the researchers selected two NSCLC cell lines (A549 and H1299) with high CircatP9A expression for further study. Subsequently, three shRNA sequences were designed to inhibit the expression of circatp9a, and an overexpression vector was designed to enhance the expression of circatp9a. The results showed that shRNA 1 and 2 could effectively inhibit the expression of circatp9a, while the overexpression vector increased its expression level. Regardless of whether CircATP9A is up-regulated or down-regulated, it does not affect the level of ATP9A mRNA. The results showed that CircatP9A silencing significantly hindered the growth, migration, and invasion of A549 and H1299 cells. The same trend was verified in the overexpression experiment.
Findings:
In summary, the results of the study show that:Circatp9A may be a promising diagnostic marker and target for non-small cell lung cancer (NSCLC).
References: Note: This article aims to introduce the progress of medical research and cannot be used as a reference for ** scheme. If you need health guidance, please visit a regular hospital.
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