In laboratory animal studies, anesthesia is a key link that not only reduces pain and discomfort in the animal, but also ensures the smooth running of the experiment. This article will introduce the common anesthesia methods for laboratory animals and their related precautions.
1. Common anesthesia methods for experimental animals.
Inhalation anesthesia method: by mixing anesthetic gas or volatile substances with oxygen, it enters the animal through the respiratory system to achieve the anesthetic effect. Commonly used inhaled substances include ether, chloroform, etc.
Injection anesthesia method: inject *** into the animal body to achieve the anesthetic effect. Commonly used injections include barbiturates, propofol, etc.
Intravenous anesthesia method: through intravenous injection into the animal's body, to achieve the anesthetic effect. Commonly used intravenous substances include sodium pentobarbital, sodium isoamobarbital, etc.
2. Relevant precautions.
Select the appropriate substance and method: according to the type, age, weight and experimental requirements of the experimental animal, select the appropriate substance and method. At the same time, it is necessary to pay attention to the dosage and use of *** substances to avoid excessive or insufficient conditions.
Strictly control the depth of anesthesia: During the experiment, it is necessary to strictly control the depth of anesthesia to avoid the situation that the depth is too deep or too shallow. Depth can lead to serious consequences such as respiratory depression and cardiac arrestShallow depth may affect the accuracy of the experimental results.
Maintain the animal's body temperature: During the anesthesia, care needs to be taken to maintain the animal's body temperature and avoid hypothermia. Hypothermia may cause adverse effects such as chills and arrhythmias in animals.
Timely treatment of adverse reactions: During the experiment, it is necessary to pay attention to the adverse reactions of animals, such as respiratory depression and cardiac arrest. In the event of an adverse reaction, it is necessary to take timely measures to deal with it to ensure the health and safety of the animal.
In short, laboratory animal anesthesia is an important part of the experimental process, and it is necessary to select appropriate substances and methods, strictly control the depth of anesthesia, maintain the animal's body temperature, and deal with adverse reactions in a timely manner. Only by doing these things well can we ensure the accuracy and reliability of the experiment and provide strong support for scientific research.