Cy7-Catalase is a probe that labels the fluorescent dye Cy7 (Cyanine 7) to Catalase (catalase). Catalase is an important enzymatic protein that catalyzes the breakdown of hydrogen peroxide (H2O2). Labeling Cy7 on Catalase can detect the distribution and activity of Catalase by fluorescent signal.
Structure: The structure of Cy7-Catalase is mainly composed of two parts: Cy7 fluorescent dye and Catalase enzyme. The Cy7 fluorescent dye is covalently bonded to the Catalase enzyme. Cy7 fluorescent dyes are typically attached to amino acid residues of catalase, such as lysine. This combination gives Cy7-Catalase a fluorescent signal that can be used for detection by techniques such as fluorescence microscopy.
Properties:1Catalytic activity: Cy7-Catalase retains the catalytic activity of Catalase, i.e., it is able to catalyze the decomposition of hydrogen peroxide. Hydrogen peroxide is a strong oxidizing agent, and too much hydrogen peroxide produced in cells can cause damage to cell structure and function, while catalase is able to effectively break it down into water and oxygen.
2.Fluorescence characteristics: Cy7-Catalase has the fluorescent properties of Cy7 fluorescent dye, and its distribution and activity in cells or tissues can be detected by techniques such as fluorescence microscopy. Cy7 fluorochrome emits fluorescence in the near-infrared region (approximately 650-750 nm) with high fluorescence intensity and low background signal.
3.Specificity: Cy7-Catalase specifically reacts with the substrate H2O2 of Catalase without reacting with other molecules. This specificity makes Cy7-Catalase an important tool for detecting and studying Catalase activity and substrate concentration.
4.Stability: Cy7-Catalase has high stability under appropriate storage conditions. In general, it should be stored in a dry, dark environment at -20°C to avoid multiple freeze-thaw cycles.
Overall, Cy7-Catalase is a probe that labels the Cy7 fluorescent dye on the Catalase, with the catalytic activity of the Catalase and the fluorescent properties of the Cy7 fluorescent dye. It can be used to detect and study the distribution, activity and substrate concentration of catalase, etc., with specificity and high stability.