Fetal bovine serum (FBS) is one of the commonly used additives in cell culture, which provides a variety of nutrients and growth factors required for cell growth. However, when using fetal bovine serum, it is necessary to ensure that the environmental conditions meet certain requirements to ensure the normal growth and culture of cells.
First, fetal bovine serum needs to be stored in a constant temperature, protected from light, and dry environment. Constant temperature ensures that the active ingredient in the serum remains stable and avoids the effects of temperature fluctuations on the serum. Protection from light can prevent the destruction of the active ingredients in the serum by ultraviolet rays and maintain the activity of the serum. A dry environment can prevent the serum from deteriorating due to moisture and maintain the quality of the serum.
Second, when using fetal bovine serum, it is important to ensure that the cell culture environment is sterile. The sterile environment can avoid the influence of bacterial contamination on cell growth and ensure the normal growth and culture effect of cells.
In addition, the use of fetal bovine serum also needs to pay attention to the following points:
1.The serum needs to be inactivated before use to remove the active ingredients and avoid interference with cell growth.
2.Serum needs to be added to the cell culture medium in the prescribed proportion to ensure the normal growth and culture effect of the cells.
3.Serum needs to be changed regularly during use to avoid bacterial contamination and nutrient depletion.
4.There may be differences in fetal bovine serum of different **, and it is necessary to choose a reliable quality product to ensure the normal growth and culture effect of cells.
In conclusion, the use of fetal bovine serum requires strict control of environmental conditions to ensure the normal growth and culture of cells. At the same time, it is also necessary to pay attention to the method of use and precautions to avoid adverse effects on cell growth.