Phosphate buffered saline solution is a commonly used chemical reagent, which is often used in biological experiments, medical testing and other fields. Proper formulation of phosphate-buffered saline solutions is critical to the accuracy and reliability of experimental results. The steps to prepare a phosphate-buffered saline solution are described below.
1. Prepare reagents and utensils.
1.Reagents: sodium phosphate monobasic (NaH2PO4), sodium phosphate dibasic (Na2HPO4), distilled water.
2.Utensils: beakers, glass rods, volumetric flasks, droppers, electronic balances.
Second, the preparation steps.
1.Calculate the amount of reagent required: Calculate the amount of sodium phosphate monobasic and sodium phosphate dibasic based on the concentration and volume of phosphate buffered saline solution required. For example, if you need to prepare 100 ml of 0For 2 mol of phosphate buffered saline solution, 016,899 g of sodium phosphate monobasic and 050,664 g of disodium phosphate.
2.Dissolve the weighed sodium dihydrogen phosphate and disodium hydrogen phosphate in distilled water respectively: dissolve the weighed sodium dihydrogen phosphate and disodium hydrogen phosphate in distilled water respectively to form two solutions of different concentrations.
3.Mix the two solutions: Mix the above two solutions of different concentrations together and stir well with a glass rod.
4.Add distilled water dropwise with a dropper: Use a dropper to add distilled water to the mixed solution until the volume of solution in the volumetric flask reaches the desired volume.
5.Shake well: Shake the solution in the volumetric flask to ensure that the reagent is fully dissolved.
6.Transfer the solution: Transfer the solution from the volumetric flask to another container for use.
3. Precautions.
1.When weighing reagents, use a precise electronic balance to ensure accurate formulation.
2.When dissolving reagents, use clean distilled water to avoid contamination.
3.When mixing two solutions, make sure they are well dissolved, otherwise the buffering effect will be affected.
4.When transferring the solution, make sure the container is dry and clean to avoid contamination.
5.The prepared phosphate-buffered saline solution should be placed in an airtight container and stored in a dry, cool place to avoid spoilage.
The above is the preparation step of the phosphate buffered saline solution. In practice, the above steps should be strictly followed to ensure that the concentration and purity of the prepared phosphate buffered saline solution meet the requirements. At the same time, for different experimental needs, the preparation concentration and volume can be adjusted according to the actual situation.