The team of Beijing Technology and Business University participated in the preparation of porcine embryonic pluripotent stem cells** animal cell cultured meat
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Domestic. The team of Beijing Technology and Business University participated in the preparation of porcine embryonic pluripotent stem cells** animal cell cultured meatOn December 13, it was reported that the preparation of animal cell cultured meat is a disruptive technology for the future food industry, which is expected to be an effective supplement to traditional animal husbandry, which is conducive to promoting environmental sustainability and agricultural carbon emission reduction, ensuring public health safety and animal welfare, and alleviating the competition for human and animal rations. More than 100 research institutions or start-ups around the world have carried out the research and development of cell-cultured meat, but there are still many challenges to realize the large-scale production of cell-cultured meat, among which the acquisition of high-quality seed cells, the research and development of animal-free media and 3D edible scaffolds, and the engineering of large-scale culture are the technical bottlenecks that need to be solved urgently in the process of cell-cultured meat industrialization. The research team of Beijing Technology and Business University participated in the establishment of a technical system for the directional induction of differentiation of porcine embryonic pluripotent stem cells into muscle serum-free, and combined with the 3D scaffold without animal-derived components, the preparation of porcine embryonic pluripotent stem cells ** animal cell cultured meat was realized for the first time. Embryonic stem cells have the ability to expand and differentiate into various tissue cells in vitro, and are ideal seed cells for cell-cultured meat. The research team further verified that the cell line can be passaged stably in vitro for a long time and maintain cell pluripotency and genome stability (more than 300 generations as of this publication), while muscle stem cells commonly used in the field of cell-cultured meat can only be passaged for 20 to 30 generations. If the passage is carried out according to the minimum proliferation ratio of embryonic stem cells at 1:3, a starting cell can obtain 330 cells at about 30 passages, which can produce all the cells equivalent to an adult pig (about 1,014 cells), and only need 50 passages to produce the equivalent of 7 billion pigs, which is 10 times the annual slaughter of pigs in China, and it only takes 100 150 days. Therefore, the pig embryonic stem cell isolation and culture technology system with independent intellectual property rights not only solves the international problem of animal stem cell construction, but also is expected to become an important chassis technology in the field of cell cultured meat industry, providing technical and resource guarantee for seizing the commanding heights of innovation and industrial development in this field.
Info**: Journal of Future Food