A brief description of the fluorescence spectrum of Sulfo Cy3 alkyne as a fluorescent probe

Mondo Fashionable Updated on 2024-01-31

Sulfo-Cy3 Alkyne as a fluorescent probe has specific fluorescence spectral properties and fluorescence stability, which are important for biolabeling and fluorescence imaging applications. (Cyananine dye derived from Star Grey).

Fluorescence spectral properties:

1.Excitation wavelength:The excitation wavelength of sulfo-Cy3 Alkyne is typically in the range of 550-560 nm. This means that at this wavelength, the probe molecule is able to absorb energy and transfer to the excited state.

2.Emission wavelength:The emission wavelength of sulfo-Cy3 Alkyne is typically in the range of 570-580 nm. At this wavelength, the probe molecule emits fluorescent photons in the process of transitioning from the excited state to the ground state.

3.Fluorescence quantum yield:Sulfo-Cy3 Alkyne typically has a high fluorescence quantum yield, which means that most of the probe molecules are able to convert to emitting fluorescent photons under excited conditions, providing a strong fluorescent signal. The high fluorescence quantum yield helps to enhance the detection sensitivity and imaging resolution of the probe.

Fluorescence stability:

1.Photostability:Sulfo-Cy3 Alkyne usually exhibits good photostability, i.e., the fluorescence intensity can be stable under continuous excitation or long-term observation. This is important for applications that require long-term live imaging or continuous monitoring to avoid fading or degradation of the fluorescent signal.

2.Photobleaching:Photobleaching of fluorescent probes refers to the gradual decrease in fluorescence intensity under prolonged exposure. Sulfo-Cy3 Alkyne typically has a slower photobleaching rate, which helps to maintain the stability and reliability of the fluorescence signal.

It is important to note that the fluorescence spectral properties and fluorescence stability of Sulfo-Cy3 Alkyne may vary depending on factors such as experimental conditions, sample environment, and purity of the fluorescent probe. Therefore, careful spectral characterization and experimental validation of fluorescent probes is recommended in applications to ensure accurate and reliable fluorescence imaging results. At the same time, the fluorescence spectral properties and stability of fluorescent probes also need to be combined with practical application requirements to select appropriate fluorescent probes.

fitc-nhs

fitc-cooh

icg-amine

icg-alkyne

icg-sulfo-osu

sulfo-cy3 alkyne

cy7.5 maleimide

sulfo-cy7 maleimide

cy7.5 cooh

Stargraydye] The above data are from the literature, and Xinggray has not been independently verified, for reference only!(The above is limited to scientific research experiments and laboratory environments).

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