SMPB Crosslinker and BMPS Crosslinker are two commonly used biological crosslinkers, which represent different types of crosslinking reagents, respectively.
1.SMPB Crosslinker (Succinimidyl 4-(P-maleimidophenyl)butyrate): SMPB Crosslinker is a kind ofActive ester crosslinking reagents, which contains a benzene ring and a butyric acid group in its chemical structure. It forms a covalent bond by reacting with amino groups in biomolecules. SMPB Crosslinker is mainly used for:Protein-proteinwithCross-linking between proteins and small molecules。It can be used with containsFree sulfhydryl group (-sh).proteins or small molecules react to form disulfide bonds (disulfide bonds) for cross-linking. This cross-linking is:Reversible, can be fractured under reducing conditions.
2.BMPS Crosslinker (Bis-Maleimido Propane): BMPS Crosslinker is one of a kindDiamidimide crosslinking reagent, which is contained in its chemical structureThere are two maleimide (maleimide) groups。It forms a covalent binding by reacting with sulfhydryl groups in biomolecules. BMPS Crosslinker is primarily used for cross-linking between protein-protein and protein-small molecules. It can react with proteins or small molecules containing free sulfhydryl groups to formCross-linking of thioether。This cross-linking is:Irreversible, not affected by reduction conditions
To sum up, the main difference between SMPB Crosslinker and BMPS CrossLincker as biological crosslinkers is the reversibility of their crosslinking forms. The SMPB crosslinker crosslinks by forming disulfide bonds, which are reversible and can be broken under reducing conditionsWhereas, BMPS Crosslinker crosslinks by forming thioether crosslinks, which are irreversible and not affected by reducing conditions. When choosing which crosslinker to use, the choice should be based on the needs of the experiment and the characteristics of the target molecule.