Bovine-melanocyte-stimulating hormone (MSH) ELISA kit.
First, the name of the product.
Bovine-melanocyte-stimulating hormone (MSH) ELISA kit.
2. Product Overview.
Bovine-melanocyte stimulating hormone (MSH) is a peptide hormone secreted by the anterior pituitary gland that promotes melanocyte proliferation and differentiation. This kit uses the principle of enzyme-linked immunosorbent assay (ELISA) to quantitatively detect the content of -melanocyte stimulating hormone (MSH) in bovine serum, plasma, tissue and other samples.
3. Product features.
1.High sensitivity: The kit uses a highly sensitive enzyme-linked immunosorbent assay to detect MSH levels down to nanograms.
2.Good specificity: This kit uses specific antibodies, which can accurately distinguish bovine MSH from other similar hormones and avoid cross-reactivity.
3.Ease of use: The kit is easy to use, requiring only one-step sample loading to complete the test, eliminating the need for cumbersome sample handling and washing steps.
4.Good repeatability: This kit has good repeatability, and the same sample can be tested multiple times, and the results are stable and reliable.
5.High reliability: This kit has undergone strict quality control procedures to ensure product quality and stability.
Fourth, the principle of detection.
This kit uses the principle of enzyme-linked immunosorbent assay (ELISA), which immobilizes specific antibodies on a microplate plate, and when added to the sample to be tested, the MSH in the sample binds to the specific antibody to form an antigen-antibody complex. An enzyme-labeled secondary antibody is then added, which binds to the antigen-antibody complex to form a sandwich structure. Finally, the substrate solution was added, and the substrate was catalyzed by enzymes to produce colored products, and the content of MSH in the sample to be measured was determined by colorimetric method.
5. Sample requirements.
This kit is suitable for the detection of bovine serum, plasma, tissue and other samples. Before use, make sure that the sample is free of contamination and hemolysis. If you have special requirements or need to process samples, please contact us before ordering.
Sixth, the operation steps.
1.Prepare the reagents: Remove all reagents from the refrigerator and bring to room temperature. Shake well before use.
2.Loading: Add the sample to be tested to the corresponding well of the microplate plate, please refer to the instructions for the amount of sample added.
3.Incubation: Incubate the plate at room temperature for a certain amount of time to allow adequate antigen-antibody binding.
4.Washing: Perform a washing operation according to the instructions to remove unbound antibodies and impurities.
5.Enzyme-labeled secondary antibody: An enzyme-labeled secondary antibody is added to the plate and binds to the antigen-antibody complex.
6.Incubation: The plate is incubated at room temperature for a certain amount of time to allow the enzyme-labeled secondary antibody to fully bind to the antigen-antibody complex.
7.Washing: Wash as required in the instructions to remove unbound enzyme-labeled secondary antibodies and impurities.
8.Substrate solution: The substrate solution is added to the microplate and the enzyme catalyzes the substrate to produce a colored product.
9.Stop the reaction: add the stop solution within the specified time to stop the enzyme-catalyzed reaction.
10.Readings: Use a microplate reader to read the absorbance value (OD value) at the specified wavelength.
7. Precautions.
1.Please read the instructions carefully and follow the instructions before using this kit.
2.This kit is intended for scientific research and teaching purposes only and should not be used for clinical diagnosis or **.
3.This kit is a one-time product, please dispose of the waste in time after use.