Protein purificationIt is a process of isolating and purifying complex mixed proteins in biological researchProtein purificationIt is a very important process, which is the basis for isolating proteins and determining their structure and function, so what is the principle of purification?Today I will give you a detailed answer.
The basic principle of purified proteins is to use the differences in the physical and chemical properties of proteins to separate mixed proteins within cells, for example, ion exchange columns use the anion exchange mode to separate ionic proteins using the charge of ions. Another common method is to isolate proteins using the different properties of protein precipitation and solubilization. For example, using the sodium sulfate precipitation method, sodium sulfate is used to act on the protein, so that the protein precipitates, and other substances do not precipitate, so that the protein is separated.
Electrophoresis is also a commonly used protein purification technology, which can separate proteins according to their molecular weight, using the force of the electric field to move the protein to different positions, and according to the molecular weight of the protein, the protein will also move different distances in the electrophoresis belt, so as to separate different proteins.
Another commonly used protein purification technique is to use the affinity of proteins to isolate proteins, which is to use the characteristics of proteins and specific affinity materials to make proteins bind to affinity materials and other substances not to isolate proteins.
Protein purificationIt is an important technology for the separation and purification of complex mixed proteins, and we need to combine the specific situation to choose when making a choice.