Dextran-PBA and HA-PBA are both commonly used materials in bioengineering, and they have certain differences in molecular structure, biocompatibility, synthesis methods, etc. In this article, we will introduce the differences between these two polymers in detail, and the direction and mechanism of their application in bioengineering.
1. Molecular structure.
Dextran-PBA is a polymer formed by esterification of dextran and phenylboronic acid (PBA). Dextran is a linear polysaccharide composed of glucose units, which has the advantages of good water solubility, non-toxicity and high biocompatibility. PBA is an organic boronic acid with specific functions that is able to react with dextran to form stable ester bonds.
HA-PBA is a polymer formed by linking hyaluronic acid (HA) and phenylboronic acid (PBA) through esterification reaction. HA is a natural linear polysaccharide composed of glucuronic acid and acetyl glucosamine with excellent biocompatibility and bioactivity. PBA is also able to react with HA to form stable ester bonds.
2. Biocompatibility.
Both dextran-PBA and HA-PBA had good biocompatibility, but HA-PBA was more biocompatible. This is because HA is a natural bioactive substance that is able to bind to receptors on the cell surface and promote cell adhesion and proliferation. In addition, HA also has the functions of regulating cytokine secretion and promoting tissue repair, so it has been widely used in tissue engineering and drug delivery.
Application direction: drug delivery field: dextran-PBA and HA-PBA can be used as carriers for drug delivery. Among them, dextran-PBA mainly uses its good water solubility and stability, as well as its ability to form complexes with a variety of drug molecules, to achieve targeted drug delivery. HA-PBA, on the other hand, can use its biological activity to bind to receptors on the cell surface to achieve precise drug delivery. In addition, HA-PBA can also play an important role in tissue engineering by regulating the secretion of cytokines and promoting tissue repair.
Tissue engineering: Both dextran-PBA and HA-PBA can be used as scaffold materials for tissue engineering. Among them, dextran-PBA has good biocompatibility and mechanical properties, which can play a role in supporting and guiding cell growth in tissue engineering. HA-PBA, on the other hand, can play an important role in tissue engineering by promoting cell adhesion and proliferation by using its biological activity. In addition, HA-PBA can also promote tissue repair and regeneration by regulating the secretion of cytokines.
Gene field: Dextran-PBA and HA-PBA can also be used as carriers of genes. Among them, dextran-PBA can form complexes with gene molecules to achieve targeted delivery of genes. HA-PBA, on the other hand, can use its biological activity to promote cell adhesion and proliferation, and improve gene transfection efficiency. In addition, HA-PBA can also promote tissue repair and regeneration by regulating the secretion of cytokines.
Cell culture field: Both dextran-PBA and HA-PBA can be used as substrate materials for cell culture. Among them, dextran-PBA has good biocompatibility and mechanical properties, which can play a role in supporting and guiding cell growth in cell culture. HA-PBA, on the other hand, can use its biological activity to promote cell adhesion and proliferation, and improve cell culture efficiency. In addition, HA-PBA can also improve the osteogenic differentiation potential of ** cells by regulating the secretion of cytokines and other mechanismsAt the same time, it can also promote osteogenic differentiation by up-regulating the expression of osteogenesis-related genes such as BMP2, Runx2, and OCN. These results provide an experimental basis for further application of HA and its derivatives in bone tissue engineering, but a large number of in vitro and in vivo experiments are still needed to verify its safety and efficacy, in order to provide experimental basis and technical guidance for clinical application.