Acrylamide is an organic compound with good chemical activity, the molecule contains a conjugated double bond, has good reactivity, can carry out various chemical reactions, such as addition reactions, polymerization reactions, etc., used to prepare plastics, adhesives, hydrogels, drug dilution systems, etc., in the field of medicine, can be used for direct reaction to prepare some anti-inflammatory drugs, or through polymers can be used as stabilizers to enhance the stability and efficacy of drugs, or in emulsified preparations, such as oil emulsions, Wax emulsions, etc., effectively help the dissolution and absorption of medicines. It is also widely used in chemical research and other fields.
However, a large number of animal experiments have proved that acrylamide has reproductive toxicity, neurotoxicity, genotoxicity and carcinogenicity, so acrylamide has become a restricted application ingredient in water treatment, cosmetics and other industry standards, such as the EU Cosmetics Law (Regulation (EC) 1223 2009) and China's cosmetics regulations have listed acrylamide as a prohibited substance in cosmetics. How to accurately qualitatively quantify acrylamide detection has become a key content in the field of detection.
At present, there are many detection methods for acrylamide, mainly gas chromatography, liquid chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry and other methods. The laboratory shared a method for detecting acrylamide content by liquid chromatography-tandem mass spectrometry as follows: using the properties of acrylamide as a polar small molecule, using a highly polar organic solvent or water as an extraction agent, the acrylamide was extracted for detection, and the appropriate column and mobile phase were selected to achieve the separation of acrylamide.
The separated compounds are fed into a mass spectrometer for detection. In mass spectrometry, an ionization source is used to convert compounds into ions, and mass analysis is performed. The presence and amount of acrylamide can be determined by measuring the specific mass-to-charge ratio (mz) ratio. Based on the data from the mass spectrometer, the amount of acrylamide can be calculated using a standard curve or other quantitative method, and an internal standard can be used for quantitative correction to improve accuracy and precision.
The high-performance liquid chromatography separation system is widely used in the detection of organic matter because of its better chromatographic resolution and sensitivity, and the combined mass spectrometry has the advantages of high sensitivity, good selectivity, easy to realize automation, etc., and the pretreatment is relatively simple, and the method with high sensitivity and selectivity can meet the needs of trace determination. The laboratory has more than 100 large-scale precision instruments such as high performance liquid chromatography-tandem mass spectrometry. It has passed CNAS and CMA metrology certification, strict quality control system, and can provide customers with customized testing solutions according to customer needs
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