Pain sensation testing methods for mouse ICH (intracerebral hemorrhage) models, including mechanical hyperalgesia test, thermal hyperalgesia test, and cold hyperalgesia test.
1. Mechanical hyperalgesia test:
1.1 Dynamic Plantar Tactile Meter:
Step 1: Place the mouse on a device fitted with a mobile probe, making sure the mouse is quiet.
Step 2: Gradually increase the force applied to the soles of the mouse feet, which can be controlled by the device.
Step 3: Record the mouse's response to applied pressure, such as foot lifting, movement, or other uncomfortable behaviors.
Step 4: Multiple tests can be tested and averaged to improve the reliability of the experimental results.
Advantages and disadvantages: Advantages: simple operation, easy to standardize, can reflect biological response.
Disadvantages: Due to environmental factors and individual animal differences, a large number of mouse samples may be required to obtain reliable results.
1.2.Vonfrey filament pain measurement:
Method: Step 1: Prepare fibers of different diameters and install them on the equipment.
Step 2: By touching the soles of the mouse feet, the diameter of the fibers can be gradually changed.
Step 3: Record the mouse's response to different fiber diameters, such as lifting the foot, cleaning the irritated area, etc.
Step 4: Perform multiple experiments and average them to improve reliability.
Advantages and disadvantages: Advantages: simple operation, easy to standardize, can reflect biological response.
Disadvantages: Due to environmental factors and individual animal differences, attention needs to be paid to the standardization of fiber diameter selection.
2. Thermal hyperalgesia test:
2.1.Plantar Thermal Tingling Meter:
Method: Step 1: Place the mouse on a glass plate, making sure the mouse is quiet.
Step 2: There is a heat source under the glass plate, and the temperature of the heat source is controlled by the device.
Step 3: Record the foot response of the mouse to the heat source stimulus, such as lifting the foot, licking the foot, etc.
Step 4: Perform multiple tests and average them to improve the reliability of the experimental results.
Advantages and disadvantages: Advantages: easy to operate, reliable results, good correlation with other thermal pain testing methods.
Disadvantages: Affected by environmental factors and individual differences, it is necessary to control the ambient temperature and other factors.
3. Cold pain hyperesthesia test:
3.1.Acetone test:
Methods: Step 1: Add acetone dropwise to the hind paw of the mouse to ensure even distribution.
Step 2: Observe the mouse's response to acetone stimuli, such as rapidly lifting, licking, or kicking.
Step 3: Perform multiple experiments and average them to improve reliability.
Advantages and disadvantages: Advantages: easy to operate, reliable results, good correlation with other cold pain testing methods.
Disadvantages: Affected by environmental factors and individual differences, it is necessary to control the concentration and amount of acetone.
3.2.Cold Plate Test:
Method: Step 1: Place the mouse on a metal plate that is kept at a constant low temperature.
Step 2: Record the duration of the mouse's response to cryogenic stimuli, such as foot lifting or other uncomfortable behaviors.
Step 3: Perform multiple experiments and take averages to improve the reliability of experimental results.
Advantages and disadvantages: Advantages: easy to operate, reliable results, good correlation with other cold pain testing methods.
Disadvantages: Affected by environmental factors and individual differences, it is necessary to control the temperature of the board and maintain the stability of the constant temperature.
3.3.Cold Plantar Test:
Method: Step 1: Use dry ice to make icicles and lightly touch the hind paws of the mouse.
Step 2: Record the duration of the mouse's response to this stimulus, such as foot lifting or other uncomfortable behaviors.
Step 3: Perform multiple experiments and take averages to improve the reliability of experimental results.
Advantages and disadvantages: Advantages: easy to operate, reliable results, good correlation with other cold pain testing methods.
Disadvantages: Due to environmental factors and individual differences, it is necessary to control the way icicles are made and touched.