Proper use of pipettesPipettes are commonly used in laboratories to measure and transfer liquids. Proper use is essential to ensure the accuracy and reliability of experimental results. The following describes the proper use of pipettes.
1.Prepare pipettes.
Before using the pipette, it needs to be washed and dried. Cleaning is done to remove impurities and residues from the tubes and improve the accuracy of the experiment. Drying is done to ensure the accuracy and reliability of the pipette.
2.Choose the right range.
Choose the pipette that is suitable for the volume of liquid you need to measure. In general, the volume of the pipette should be larger than the volume of liquid that needs to be transferred.
3.Rinse the pipette.
Before using the pipette, it needs to be rinsed with water. This removes residual moisture and impurities, ensuring the accuracy of experimental results.
4.Aspirate liquids.
Insert the pipette into the liquid, making sure that the bottom of the tube is completely submerged in the liquid. Then gently pull out the pipette to allow the liquid to enter the tube. Be careful not to fill the tube with air so as not to affect the experimental results.
5.Transfer liquids.
Remove the pipette from the liquid and place it vertically for a period of time to allow the liquid to flow to the nozzle. Gently aspirate the liquid from the pipette mouth with filter paper to make sure there is no residual liquid. Next, insert the pipette into the receiving vessel and gently pull out the pipette to allow the liquid to flow into the container.
6.Wash the pipette.
After using the pipette, it needs to be cleaned promptly. Rinse the remaining liquid in the tube with clean water first, and then dry it with clean filter paper. Finally, place the pipette in a dry and ventilated place to dry.
In summary, the correct use of pipettes requires the following considerations: choosing the right volume, rinsing the pipette, aspirating the liquid, transferring the liquid, cleaning the pipette, etc.