How to do fatty acid experiments?

Mondo Health Updated on 2024-02-22

Fatty acid is a compound composed of carbon, hydrogen and oxygen, and is a general term for fatty acid carboxylic acid compounds formed by the hydrolysis of natural oils. According to the length of the carbon chain, it can be divided into short-chain fatty acids - the number of c atoms on the carbon chain is less than 6; Medium-chain fatty acids - the number of c atoms on the carbon chain is 6-12; Long-chain fatty acids – The number of atoms on the carbon chain is greater than 12. According to the degree of saturation, it can be divided into saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids. Fatty acids are important components of cell membrane phospholipids, which can directly regulate cell membrane composition, proteins and intramembrane receptors. At present, more than 200 naturally occurring fatty acids have been found in plants and animals.

Customer** orders - orders Confirmation of experimental materials - extraction of target metabolites - GC-MS targeted data acquisition - qualitative analysis of metabolites - Quantitative analysis - Bioinformatics analysis - Result delivery.

Detection metrics

Our advantages

1. Wide detection range - dozens of fatty acid metabolites can be detected;

2. High sensitivity - GC-MS mass spectrometer is used for data acquisition;

3. Strict quality control and accurate results;

4. Professional service - with professional experimental technicians and pre-sale, in-sale and after-sales service team.

Applicable scenarios

1. Biomarker screening;

2. Research on metabolic pathways and related functional genes;

3. Metabolism-related physiological and pathological research;

4. Molecular functional research of diseases;

5. Evaluate drug toxicity;

6. Research on plant stress resistance mechanism and growth and development mechanism;

7. Plant microbial interaction and medicinal plant research.

Experimental cycle

35 working days from the time of sample delivery.

Sample Acceptance Criteria

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1. Sample**, content, status and other basic information;

2. Abundant relevant materials and literature as much as possible.

During the experiment, the customer can log in to the management system at any time to view the real-time progress of the project. When the experiment is completed, the system will automatically notify the customer via SMS and send the experiment report to view**. After the experiment is completed, the test report and test results can be viewed or printed and permanently retained.

1. Complete final report;

2. Experimental raw data;

3. The results of bioinformatics analysis include:

A. Data quality control analysis (QC, RSD);

b. Differential analysis of metabolites: (univariate statistical analysis of T test, PCA, OPLS-DA, differential metabolite heat map, differential metabolite volcano map, Wayne diagram, differential metabolite VIP map, etc.);

c. Functional annotation analysis: hierarchical clustering analysis, KEGG annotation analysis, KEGG enrichment analysis;

d. Advanced data analysis: regression analysis, trend cluster analysis, random forest analysis, ROC analysis.

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