DNMT1 is a DNA methyltransferase that belongs to the methyltransferase family and is a key enzyme in maintaining DNA methylation homeostasis. In the process of DNA methylation, DNMT1 is responsible for transmitting methylation information in the cell**, ensuring the transmission of DNA methylation patterns in daughter cells. Therefore, DNMT1 plays an important role in cell proliferation, differentiation, and development. Catalog No. PA1000-7730
The structure of the DNMT1 protein contains an N-terminal methyltransferase domain and a C-terminal DNA-binding domain. The methyltransferase domain achieves selective methylation of DNA by binding to cofactors, while the DNA binding domain can bind to DNA to ensure that methylation information is transmitted in cells**. In addition, DNMT1 also contains a conserved CXCc domain, which can bind to the unmethylated CpG site upstream of DNA and participate in the regulation of methylation.
The role of DNMT1 in biological function is mainly manifested in the following aspects:
1.Maintain genome homeostasis: DNMT1 maintains genome stability by replicating methylation information during DNA synthesis to ensure the stability of DNA methylation levels.
2.Cell proliferation and differentiation: DNMT1 plays an important role in cell proliferation and differentiation. During cell proliferation, DNMT1 ensures the transmission of daughter cell DNA methylation patterns and ensures the normal function of cells**. In cell differentiation, DNMT1 can regulate the expression level of genes and affect the differentiation direction of cells.
3.Disease occurrence and progression: DNMT1 also plays an important role in the occurrence and progression of a variety of diseases. For example, DNMT1 plays a key role in its occurrence and progression, influencing cell proliferation and metastasis by regulating the methylation state of repressor genes.
In recent years, scientists have also begun to use recombinant DNA technology to engineer DNMT1 to prepare recombinant proteins with specific functions. By modifying the structure and function of DNMT1 protein, scientists hope to be able to study its mechanism of action in cell proliferation, differentiation and disease more deeply, and provide new ideas for diseases and genes.
Overall, DNMT1 is an important DNA methyltransferase that plays an important role in cell proliferation, differentiation, and disease. The study of recombinant DNMT1 provides a new way for us to understand its function and mechanism, and helps to reveal its potential application value in diseases and genes.