Single-cell sequencing technology solves the problem of cell heterogeneity and greatly improves the resolution of our understanding of living systems, and has become a routine method for life science research. MGI's single-cell platform has accumulated a certain practical foundation in scientific research and application, covering a variety of research and application fields, such as:Tumor research, cell profiling, brain science, etc.
As of now,Based on MGI's single-cell DNBELAB C series products (3'RNA and ATAC), a total of 65 SCI** articles have been published. Among them, 36 articles will be published in 2022 (including 2 articles in the main journal of Nature), 21 articles will be published in 2023 (including 2 articles in the main journal of Cell), and 2 articles will be published in 2024, with a cumulative impact factor of more than 1000 points.
MGI's single-cell platform covers a wide range of fields in terms of scientific research and application, especially in the field of scientific research and applicationHe has made outstanding contributions to tumor research, cell mapping, brain science, immunology and other fields。The species studied in the article, except for the largest number of humans and rats, accounted for 338% and 22In addition to 1%, 14 other species (e.g. monkeys, zebrafish, ants, Arabidopsis thaliana, etc.) were also involved. The article covers not only monoomics (RNA or ATAC) applications, but also multi-omics joint analysisThere are 9 articles on the combined analysis of DNBELAB c-3'RNA and DNBELAB c-ATAC with multi-omics single-cell technology, and 8 articles on the combined analysis of DNBELAB c-3'RNA and Stereo-Seq with spatiotemporal omicsThe related research has a trend of multimodality, refinement and three-dimensionalization, indicating that DNBELAB C series products have played a key role in the scientific research in the field of single cells, and helped scientists to explore in related scientific research fields.
Innovative and intelligent manufacturing tools help single-cell omics
Enter the era of standardization and scale
Since MGI launched the first DNBELAB C4 single-cell portable platform in China in 2019, it has successfully launched three generations of single-cell 3' RNA library preparation kits. Currently, single-cell 3'RNA-V30 The product is being trialed by partners and has received wide acclaim, the latest version of the product is the result of five years of careful research and development and improvement, using a unique multi-magnetic bead trapping cell system with excellent performance.
The productThe capture efficiency is stableaboveSample labels are not usedIn this case, a maximum of a single slide is acceptableto capture10,000 cells, useSample labels, product markingsMultiplexed sequencing can be performed, up to the mostA single channel can be captured10,000 cellstoHuman peripheral blood mononuclear cells(pbmc)and mouse brain cell nucleiSamples are averaged per cell for two sample typesMedian gene testAll can be achievedmore than that；These breakthrough optimizations mark that MGI's technology and application in the field of single cells have reached a new height, providing powerful tools for biomedical research, especially in precision medicine and complex biological systems.
Human PBMCs, 20,000 cells were put in, and the median number of genes was 2763.
Mouse brain nuclei, 20,000 nuclei were invested, and the median number of genes was 2657.
In addition, MGI has also laid out single-cell omics library construction productsSingle-celledatacLibrary preparation kit, single cellcut&tagThe library construction kit is a portable one based on droplet microfluidic technology in hardwarec4system and independent multi-channeltaim 4(TarzanSingle-cell droplet generation instruments are available;
Portable C4 and stand-alone multichannel TAIM 4, single-cell library preparation kits.
MGI's large-scale raw material production and processing capabilities have greatly reduced the cost of single-cell sequencing technology, enabling researchers to enter the era of single-cell research with a lower thresholdIn addition, MGI's high-throughput sequencing, automated sample processing and library constructionbitThe layout of data processing products and the leading technology of BGI spatiotemporal omics have formed a complete and comprehensive one-stop platform and cutting-edge laboratory technology solutions for the layout of cytomicsPromote the large-scale and standardized production of single-cell data by more researchers.
MGI full-process single-cell products.
DCS Multiomics Laboratory.
In addition, MGI focuses on the construction of an ecological chain for the popularization and application of single-cell sequencing technology, and in 2023, three new certified service providers will be added: Harbin Nebula Biotechnology Co., Ltd., Wuhan Aiji Baike Biotechnology Co., Ltd., and Huazhi Biotechnology Co., Ltd., bringing the total number of certified single-cell DNBELAB C series products services to 12 companies in China.
In 2023, three new single-cell DNBELAB C series product certification service providers will be added.
In 2023Based on MGI's DNBELAB C series
Research published on a single-cell platform**
1. single-cell spatial transcriptome reveals cell-type organization in macaque cortex
doi：
Journal:cell（if=64.5）
Published by:Center for Excellence in Brain Science and Intelligent Technology, Chinese Academy of Sciences, Shenzhen BGI Life Science Research Institute, etc.
Subjects:Cynomolgus monkey brain, snrna—1,493,240 nucleus, stereo-seq—161 slices.
Use a technique or method:Tissue embedding and sectioning, immunohistochemical staining, RNA in situ hybridization, Stereo-Seq, DNbeLab C SN3'rna
Sequencing Platform:dnbseq-tx
Article Research Highlights:
A comprehensive and complete classification method of cynomolgus monkey cerebral cortex cell types was established. Stereo-Seq technology was used to reveal the global distribution of 264 cell types in the cerebral cortex, and the specific marker genes for each cell type were indexed. The study found that different cell types in brain regions had different densities, compositions, and relationships with cerebral cortex levels. Through cross-species analysis, primate-specific cell types were found to be predominantly enriched in the fourth layer of the brain, providing an in-depth understanding of the structure and function of the primate brain. 2. live birth of chimeric monkey with high contribution from embryonic stem cells
doi：
Journal:cell（if=64.5）
Published by:The Center for Excellence in Brain Science and Intelligent Technology of the Chinese Academy of Sciences, the Shanghai Brain Science and Brain-like Research Center and many other units have cooperated.
Subjects:Embryonic stem cells, PBMCs, brain, heart, liver, bone marrow, snrna—50,812 nucleus, scrna—5,230 cells
Use a technique or method:Teratoma formation, in vitro embryo culture, embryo transfer, immunofluorescence and AP staining, RT-qPCR, mitochondrial metabolism, STR, SNR and karyotyping, flow cytometry, DNBELAB C SC3'RNA and SN3'RNA, SmartSeq2 single-cell technology, WGS, methylation, RNA high-throughput sequencing.
Sequencing Platform:bgiseq-500、dnbseq-t7、dnbseq-tx、abi prism 3730、novaseq 6000
Article Research Highlights:
Embryonic stem cells in monkeys under different pluripotency states were comprehensively characterized.
The chimeric culture process was optimized to improve the penetration rate of embryonic stem cells in the host embryo.
A strict evaluation system was established, and chimeric monkeys with a high proportion of embryonic stem cell contributions were successfully obtained.
Monkey embryonic stem cells can differentiate into germ cells and placenta in chimeric monkeys.
3. an invasive zone in human liver cancer identified by stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression
doi：
Journal:cell research（if=46.297）
Published by:China Precision Medicine Center, Shenzhen BGI Life Science Research Institute and many other units.
Subjects:Human liver cancer and next to liver cancer, snRNA—33, 111 nucleus, stereo-seq—98 sections.
Use a technique or method:IF and IHC staining, flow cytometry, ELISA, Stereo-Seq, DNBELAB C SN3'rna，bulk rna seq
Sequencing Platform:dnbseq-tx、novaseq 6000
Article Research Highlights:
The spatial transcriptional heterogeneity of T, P, M and normal or metastatic LN in human primary hepatocellular carcinoma was revealed.
It was determined that there was an immunosuppressive microenvironment around the tumor invasion boundary, and immune cells were significantly enriched in this region.
It was found that the activation of JAK-stat3 in the invasion region induced the high expression of the damaged hepatocyte subtype SaaS.
The SAA+ hepatocyte subtype contributes to macrophage recruitment and M2 polarization, thereby influencing tumor development.
4. a single-cell atlas of west african lungfish respiratory system reveals evolutionary adaptations to terrestrialization
doi：
Journal:nature communications（if=17.694）
Published by:University of Chinese Academy of Sciences, University of Copenhagen and other units.
Subjects:Lungour gills, lungs, scrna—140,952 cells
Use a technique or method:fish，dnbelab c sc3'rna，10x genomics sc3'rna
Sequencing Platform:mgiseq-2000、dnbseq-tx
Article Research Highlights:
Single-cell transcriptional profiles of lungs and gills of West African lungfish under freshwater and terrestrial conditions were revealed;
During the adaptation of the African lungfish to terrestrialization, the cell type remained unchanged and the metabolic activity of respiration-related lung and gill cells was down-regulated, even during short-term summer hibernation.
Through cross-species comparative analysis, it was found that the lungs of lungfish and the respiratory organs of higher mammals had homology in terms of cell composition and key genes.
5. interferon stimulated immun eprofile changes in a humanized mouse model of hbv infection
doi：
Journal:nature communications（if=17.694）
Published by:The Eighth Affiliated Hospital of Guangzhou Medical University, Institute of Biophysics, Chinese Academy of Sciences and other units.
Subjects:mouse PBMC, heart, liver, spleen, lung, kidney, muscle, brain.
Use a technique or method:Plasmid construction, CRISPR Cas9, flow cytometry, DNBELAB CS3'rna，bulk rna seq
Sequencing Platform:mgiseq-2000，dnbseq-t7，novaseq 6000
Article Research Highlights:
CRISPR Cas9 technology was used to successfully establish a human-derived receptor mouse model that could realistically simulate interferon response in humans.
It was found that the immune activation response of PEG-IFN 2 to PBMCS in Huifnar mice was similar to that in human PBMCs.
It comprehensively depicts the transcriptome response map of multiple organs stimulated by human interferon;
Human PEG-IFN2** significantly reduces HBSA** in Huifnar mice.
6. gdf11 slows excitatory neuronal senescence and brain ageing by repressing p21
doi：Journal:nature communications（if=17.694）
Published by:Zhejiang University School of Medicine, Affiliated Run Shaw Hospital, Fudan University Translational Institute of Brain Science and other units.
Subjects:Mouse, marmosets, human brain, snrna—24,803 nucleus
Use a technique or method:OFT, PAT, NORT, EPM, 3CT, TST test, plasmid construction, CRISPR Cas9, ChIP-qPCRC, SA--gal, immunofluorescence, immunohistochemical staining, transmission, immunoelectron microscopy, DNBELAB C SN3'rna，bulk rna seq
Sequencing Platform:dnbseq-tx，dnbseq-t7，novaseq 6000
Article Research Highlights:
The highly specific expression of growth differentiation factor 11 (GDF11) in the central nervous system, especially in excitatory neurons, was confirmed, and the expression pattern of GDF11 was found to be significantly conserved in mouse, marmosets and human brains.
Knockout of GDF11 in excitatory neurons at the local and whole brain levels weakened the cognitive and memory functions of mice, suggesting that GDF11 plays an important role in the maintenance of neurological function.
The knockdown of GDF11 leads to the upregulation of PSMAD2 and SMAD3, which in turn promotes the increase of P21 expression, which may induce cells to enter a senescent state.
GDF11 is able to delay the aging process of excitatory neurons and the brain by inhibiting the expression of P21, improve the aging of cognitive function, and maintain the lifespan of mice. This finding provides important clues for understanding the mechanisms of brain aging and finding potential anti-aging** strategies.
7. single cell multi-omics reveal intra-cell-line heterogeneity across human cancer cell lines
doi：Journal:nature communications（if=17.694）
Published by:Southern University of Science and Technology, Shenzhen BGI Life Science Research Institute and other units.
Subjects:40 human cancer cell lines, scrna—23,089 cells, scatac—54,597 cells
Use a technique or method:Virus packaging and transduction, DNBELAB C SC3'rna，dnbelab c scatac，bulk rna seq
Article Research Highlights:
Single-cell transcriptome and epigenomic sequencing revealed heterogeneity in gene regulatory networks within cancer cell lines, a finding that helps to understand the complex biology within cancer cells;
The interaction between the single-cell transcriptome and the epigenome was analyzed, which is critical for understanding cell state and gene regulatory networks;
The transcriptional dynamics during cell line proliferation were observed, which provided a new perspective for studying the proliferation mechanism of cancer cells.
The discovery that the hypoxic environment can drive the response of cancer cell heterogeneity is of great significance for studying cell behavior in the tumor microenvironment.
single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment
doi：
Journal:nature communications（if=17.694）
Published by:Institute of Zoology, Chinese Academy of Sciences, CITIC Xiangya, Third Hospital of Beijing Medical University and other units.
Subjects:human **, PBMC, scrna—21,489 cells, scatac—24, 005 cells;
Use a technique or method:Immunofluorescence, immunohistochemical staining, cell migration, invasion, flow cytometry, 10x SC3'rna，dnbelab c scatac，cut&tag，10x visium；
Sequencing Platform:dnbseq-tx，novaseq 6000
Article Research Highlights:
Successful identification of a common key gene expression program between seminoma and blast germ cells. This discovery provides a new perspective on understanding the biology of these two cell types;
Fifteen different immune cell subtypes were identified in the tumor microenvironment (TME), and combined with spatial transcriptomics studies, it was found that immune cell subtypes with depletion characteristics were more likely to be located close to the tumor region. This is of great significance for understanding the distribution and function of immune cells in the tumor microenvironment;
The function of the transcription factor TFAP2C in promoting tumor invasion and metastasis was in-depth characterized. This study provides a possible new target for the best tumor strategy;
A multi-omics map of the microenvironment of human seminoma in situ was established, which provides a valuable data resource for understanding the complexity of the microenvironment of seminoma.
cancer-associated fibroblasts undergoing neoadjuvant chemotherapy suppress rectal cancer revealed by single-cell and spatial transcriptomics
doi：
Journal:cell reports medicine（if=14.3）
Published by:The Sixth Affiliated Hospital of Sun Yat-sen University, South China University of Technology and other units.
Subjects:Human rectal cancer, scrna—33, 3285 cells, stereo-seq—4+ sections.
Use a technique or method:dnbelab c sc3'rna，stereo-seq
Sequencing Platform:dnbseq-tx
Article Research Highlights:
The remodeling of cancer-associated fibroblast (CAFS) populations by neoadjuvant chemotherapy is closely related to the ** response. This finding has important implications for understanding the mechanism of action of chemotherapy in cancer**;
The study found that the remodeled cancer-associated fibroblast subsets were able to activate the immune response through spatial recruitment and tandem regulation, and inhibit tumor progression through a variety of cytokines.
In particular, CAF FAP upregulates the epithelial-mesenchymal transition (EMT) of malignant cells through the induction of MIR4435-2HG, resulting in a deterioration in the prognosis of patients, a result that provides a new potential target for rectal cancer.
10. stem cell competition driven by the axin2-p53 axis controls brain size during murine development
doi：Journal:developmental cell（if=13.417）
Published by:Institute of Genetics and Developmental Biology, Chinese Academy of Sciences.
Subjects:Mouse brain, scrna—18,951 cells
Use a technique or method:Plasmid construction, galactosidase, immunohistochemical staining, in situ hybridization, virus packaging and transduction, bulk RNA-seq, flow cytometry, western blotting, co-immunoprecipitation, DNBELAB c scrna, Smart-Seq2, bulk RNA-seq;
Sequencing Platform:dnbseq-tx，hiseq2500
Article Research Highlights:
It revealed that there is endogenous cell competition between neural stem cells (NPCs). This discovery has important implications for understanding the biology of neural stem cells;
It was found that in the chimeric environment, axin2-deleted stem cells were eliminated, while p53-mutant stem cells were significantly expanded, which provides a new perspective for understanding the molecular mechanism of stem cell competition.
It has been confirmed that the competition process of neural stem cells plays a key role in regulating the size of the brain, and this discovery has important scientific value for the regulatory mechanism of brain development.
single-nucleus chromatin landscapes during zebrafish early embryogenesis
doi：Journal:scientific data（if=9.8）
Published by:Shenzhen BGI Life Science Research Institute, Huazhong Agricultural University and other units.
Subjects:Zebrafish embryos, scrna—62,699 cells, scatac—51,620 cells
Use a technique or method:dnbelab c scatac
Sequencing Platform:dnbseq-tx
Article Research Highlights:
SNATAC-Seq maps were successfully established at different time points on the first day of development of zebrafish embryos, which provided valuable gene regulatory information for the study of early embryonic development.
Data analysis showed a good correlation between gene activity scores from SNATAC-SEQ data and gene expression values from SCRNA-SEQ data, a finding that validates SNATAC-Seq as an effective tool to study gene expression regulation.
a scatac-seq atlas of chromatin accessibility in axolotl brain regions
doi：Journal:scientific data（if=9.8）
Published by:Shenzhen BGI Institute of Life Sciences, Southern Medical University.
Subjects:Salamander brain, scatac—81,199 cells
Use a technique or method:dnbelab c scatac
Sequencing Platform:dnbseq-tx
Article Research Highlights:
The Scatac-seq chromatin accessibility map of the salamander brain was successfully mapped, which provides a valuable resource for understanding the genetic regulation of the salamander brain.
Scatac-Seq analysis revealed a unique set of regulatory elements unique to each cell type; This finding has important implications for understanding cell-specific gene regulation in the brain of axolotls.
13. deciphering the distinct transcriptomic and gene regulatory map in adult macaque basal ganglia cells
doi: Journal:giga science（if=9.2）
Published by:BGI Life Science Research Institute.
Subjects:Cynomolgus monkey brain, SCRNA—101,431 cells, SCATAC—170,608 cells
Use a technique or method:dnbelab c sc3'rna，dnbelab c scatac
Sequencing Platform:dnbseq-tx
Article Research Highlights:
Using scRNA and scatac technology, the data of more than 270,000 single cells were analyzed, and a fine single-cell map of the basal ganglia of cynomolgus monkeys was constructed.
In the basal ganglia, neuronal and non-neuronal cells exhibited distinctly different patterns of gene expression and epigenetic regulation.
A panel of astrocytes associated with neurodegenerative diseases and specific in the basal ganglia of cynomolgus monkeys were identified;
Combined with the epigenetic landscape of basal ganglion cells in cynomolgus monkeys and human disease genetics, a regulatory module consisting of cis-regulatory elements associated with psychiatric disorders was identified. This finding provides a new perspective for understanding the gene regulatory mechanisms associated with psychosis.
14. a single-cell transcriptome atlas of pig skin characterizes anatomical positional heterogeneity
doi：
Journal:elife（if=8.713）
Published by:Sichuan Agricultural University and other units.
Subjects:Porcine ** (head, ears, shoulders, back, abdomen and legs), scrna—233,715 cells
Use a technique or method:Immunofluorescence staining, DNBELAB C SC3'rna
Sequencing Platform:dnbseq-tx
Article Research Highlights:
The single-cell transcription profiles of 6 anatomical parts (head, ears, shoulders, back, abdomen, and legs) of different adult pigs were constructed, which provided comprehensive cell-level data for the study of biology.
The specific gene expression patterns associated with anatomical location and cultivar were revealed, as well as their biological functions in the immune response and the synthesis of extracellular matrix (mainly collagen);
Pigs were found to be very similar to humans in terms of cell type composition and gene expression patterns, suggesting that pigs can be used as an effective model for studying humans, especially at the single-cell level.
15. stemness-related genes revealed by single-cell profiling of naïve and stimulated human cd34+ cells from cb and mpb
doi：Journal:clin. transl. med.（if=8.554）
Published by:University of Chinese Academy of Sciences, Shenzhen Children's Hospital and other units.
Subjects:Umbilical cord blood, mobilized peripheral blood, scrna—16,196 cells
Use a technique or method:Magnetic bead-enriched cells, flow cytometry, DNBELAB CS3'rna
Sequencing Platform:dnbseq-tx
Article Research Highlights:
The transcriptional profiles of primitive in vitro cultured CD34+ cells in human umbilical cord blood and mobilized peripheral blood were analyzed in detail.
The differentiation trajectories of all cells were mapped using monocle software, and 7 branches were found, most of which were hematopoietic stem cell pluripotent precursor cells located near the tip of the trajectories;
CS-SRG (umbilical cord blood-specific stem cell-related genes) and CT-SRG (mobilization peripheral blood-specific stem cell-related genes) share rich signaling pathways, and the translation and processing of dynamic proteins may be common requirements for maintaining stem cell properties.
Small-scale drug screening for CT-SRG was carried out using CMAP software, and it was found that cucurbitacin I can effectively enhance the in vitro expansion of hematopoietic stem cells while maintaining their stem cell properties, which provides new possibilities for the application and ** of hematopoietic stem cells.
16. single-cell and spatial transcriptomics reveal postn+ cancer-associated fibroblasts correlated with immune suppression and tumour progression in non-small cell lung cancer
doi：
Journal:：clin. transl. med.（if=8.554）
Published by:Shenzhen, Peking University, Shenzhen Hospital and other units.
Subjects:Human lung cancer, parapulmonary cancer, distal lung normal tissue, scrna-16, 2 036 cells, stereo-seq-15+ sections.
Use a technique or method:IHC, miHC staining, DNBELAB C SC3'rna，stereo-seq
Sequencing Platform:dnbseq-tx
Article Research Highlights:
In advanced non-small cell lung cancer (NSCLC), Postn+ carcinoma-associated fibroblasts (CAFS) were enriched, and gene expression characteristics related to extracellular matrix remodeling, tumor invasion and immunosuppression were exhibited.
Postn+ CAFs are closely mapped to SPP1+ macrophages and correlate with a decrease in T cells with a depleted phenotype, suggesting the interaction and immunosuppressive mechanisms of these cells in the tumor microenvironment.
The expression of POSTN and POSTN+ CAFs were identified as important prognostic factors for non-small cell lung cancer, providing new clues for biomarker research and target development of lung cancer.
. uncovering the prominent role of satellite cells in par**ertebral muscle development and aging by single-nucleus rna sequencing
doi：Journal:genes & diseases（if=7.376）
Publication sheetBits:The University of Hong Kong-Shenzhen Hospital, Qingdao BGI Research Institute and other units.
Subjects:Mouse paravertebral muscles, scrna—34,589 cells
Use a technique or method:dnbelab c sn3'rna
Sequencing Platform:dnbseq-g400
Article Research Highlights:
A single-cell transcription** profile of paravertebral muscles covering all stages from mouse embryonic to old age was successfully constructed. This provides detailed cell-level insights into the development and aging of paravertebral muscles;
Combining 69 public datasets and the data from this study, a single-cell database of skeletal muscle was established. This database provides an invaluable resource for studying the development, function, and disease of skeletal muscle.
18. cell atlas of ccl4-induced progressive liver fibrosis reveals stage-specific responses
doi：Journal:zoological research（if=6.975）
Published by:Jilin University, Guangzhou Medical University and other units.
Subjects:Mouse liver, scrna: 49,919 cells
Use a technique or method:Immunofluorescence, single-molecule fluorescence in situ hybridization and histological staining, DNBELAB C SN3'rna
Sequencing Platform:dnbseq-tx
Article Research Highlights:
A dynamic single-cell nuclear transcriptional profile of the progressive development of liver fibrosis in mice induced by carbon tetrachloride was constructed. This provides a new perspective for understanding the cellular mechanisms of liver fibrosis;
Molecular roadmaps of hepatic stellate cell activation at different stages of liver fibrosis were revealed. This finding is critical for understanding the critical role of hepatic stellate cells in liver fibrosis;
The dynamic changes of angiogenesis response in different stages of liver fibrosis were observed, which increased the understanding of the role of angiogenesis in liver pathology.
Cell-cell communication networks and specific gene regulatory networks during progressive liver fibrosis were analyzed, which may be important for the development of new strategies.
18. cell atlas of ccl4-induced progressive liver fibrosis reveals stage-specific responses
doi：Journal:zoological research（if=6.975）
Published by:Jilin University, Guangzhou Medical University and other units.
Subjects:Mouse liver, scrna: 49,919 cells
Use a technique or method:Immunofluorescence, single-molecule fluorescence in situ hybridization and histological staining, DNBELAB C SN3'rna
Sequencing Platform:dnbseq-tx
Article Research Highlights:
A dynamic single-cell nuclear transcriptional profile of the progressive development of liver fibrosis in mice induced by carbon tetrachloride was constructed. This provides a new perspective for understanding the cellular mechanisms of liver fibrosis;
Molecular roadmaps of hepatic stellate cell activation at different stages of liver fibrosis were revealed. This finding is critical for understanding the critical role of hepatic stellate cells in liver fibrosis;
The dynamic changes of angiogenesis response in different stages of liver fibrosis were observed, which increased the understanding of the role of angiogenesis in liver pathology.
Cell-cell communication networks and specific gene regulatory networks during progressive liver fibrosis were analyzed, which may be important for the development of new strategies.
19. single-cell and spatiotemporal transcriptomic analyses reveal the effects of microorganisms on immunity and metabolism in the mouse liver
doi：Journal:computational and structural biotechnology journal（if=6）
Published by:Shenzhen BGI Institute of Life Sciences, Huazhong Agricultural University.
Subjects:Mouse liver, scrna—14,586 cells, snrna—21,947 nucleus, stereo-seq
Use a technique or method:dnbelab c sc3'rna、sn3'RNA, Stereo-Seq, untargeted metabolomics, immunofluorescence staining.
Sequencing Platform:dnbseq-t7，dnbseq-tx
Article Research Highlights:
Detailed single-cell and sterial-transcriptional profiles of sterile and specific pathogen-free mouse livers were constructed. This study provides a basis for understanding the biological changes of the liver under the influence of microorganisms;
Stereo-Seq data were used to confirm the microbial-mediated accumulation of Kupffer cells in the periportal region.
Stereo-Seq technology accurately divides the liver lobules into 8 layers and 3 modes according to the gene expression levels at different levels, increasing the understanding of liver structure and function.
Non-targeted metabolism experiments in the liver showed a significant decrease in propionate levels in germ-free mice, which may be related to the regulation of genes related to bile acid and fatty acid metabolism, providing new insights into the interaction between gut microbiota and liver metabolism.
microbiota-mediated shaping of mouse spleen structure and immune function characterized by scrna-seq and stereo-seq
doi：Journal:journal of genetics and genomics（if=5.723）
Published by:Shenzhen BGI Life Science Research Institute, Sun Yat-sen University, etc.
Subjects:Mouse spleen, scrna—33,572 cells, stereo-seq—8 10 sections.
Use a technique or method:dnbelab c sc3'rna，stereo-seq
Sequencing Platform:dnbseq-tx
Article Research Highlights:
ScRNA-seq datasets using sterile and pathogen-specific free (SPF) mice reveal multiple immune landscapes in the spleen. This provides a new perspective for understanding the immune function of the spleen;
It shows how the microbiota shapes the anatomy and immune function of the mouse spleen. This discovery is critical for understanding the interaction of the microbiota with the host immune system;
The discovery of a disturbed spleen stratification in mice lacking microbiota reveals the critical role of microbiota in maintaining spleen structure and functional stability.
21. single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis
doi：Journal:frontiers in neuroscience（if=4.3）
Published by:Hangzhou BGI Life Science Research Institute.
Subjects:Mouse embryos, SCATAC: 101,599 cells
Use a technique or method:dnbelab c scatac, scatac and scrna, scrna, and stereo-seq data integration analysis.
Sequencing Platform:dnbseq-t7，dnbseq-tx
Article Research Highlights:
A dynamic single-cell ATAC (SCATAC) landscape across developmental trajectories during mouse somatic cytogenesis was constructed, revealing an important cell type-specific regulatory network in organogenesis.
Particular attention is paid to the characterization of cell type-specific regulatory profiles in the spinal cord lineage, as well as the reconstruction of developmental trajectories. This provides a new perspective for understanding spinal cord development;
These data have been used to identify cell type-related traits and potential disease linkages during embryonic development, providing an important basis for future research.
Additional: Published articles on DNBELAB C series single-cell platform over the years