Product name: Fluorescein FITC-labeled transferrin.
Product Name: fitc-transferrin
Product name: Fluorescein FITC labeled bovine serum albumin.
Product Name: FITC-BSA
Product name: Cyanine dye Cy5 labeled bovine serum albumin.
Product Name: CY5-BSA
Product name: Cyanine dye cy5 labels human serum albumin.
Product Name: CY5-HSA
*Business: Chongqing Xinweichuang.
IRB-NHS can be labeled with near-infrared fluorophores to functional molecules such as antibodies, proteins, peptides, and biopolymers under physiological conditions. After labeling, the dynamic distribution of target functional molecules in vivo and the targeting efficiency of lesions can be observed non-invasively by the small animal optical imaging system. Features: good water solubility, high absorbance and quantum yield, stable photochemical properties, easy labeling.
Experimental tools Raw materials.
1. Active near-infrared fluorescent fluorescent probe (IRB-NHS) detection kit.
2. PBS phosphate buffer solution.
3. DMF4, shaker.
5. Ultrafiltration tube.
The antibodies, peptides, and proteins to be labeled are dissolved in PBS phosphate buffered solution with an adjusted pH of 74。The IRB-NHS active near-infrared fluorescent fluorescent probe was dissolved in 100 ul of DMF and added dropwise to the dissolved solution. Return the above mixture to the shaker and react for 2 h.
After the reaction, the reaction solution was added to the ultrafiltration tube for ultrafiltration (4000 rpm, 30 3 min), and the corresponding solid powder labeled with IRB was lyophilized by lyophilizing the solution on the upper layer of the ultrafiltration tube.
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Transferrin