The three key elements of good cell cultivation are: good cells**, correct experimental practices, and a suitable culture system. Among them, the "appropriate culture system" is to create an environment suitable for cell growth and establish a satisfactory "home" for cells.
Basic conditions for cell culture:
1.Suitable cell culture media
Appropriate cell culture medium is one of the important conditions for cell growth and proliferation in vitro, which not only provides cell nutrition and basic materials to promote cell growth and proliferation, but also provides a living environment for the growth and reproduction of cultured cells.
2.High-quality serum
Currently, most synthetic media require the addition of serum. Serum is one of the important components of cell culture media, containing a variety of growth factors and other nutrients required for cell growth.
3.Sterile, non-toxic cell culture environment
A sterile, non-toxic operating environment and culture environment are the primary conditions to ensure the success of cell culture in vitro. Cells cultured in vitro lack the ability to defend against microorganisms and toxic substances, and once they are contaminated with microorganisms or toxic substances, or accumulate their own metabolites, they can lead to cell poisoning and death. Therefore, when culturing cells in vitro, it is necessary to keep the cell living environment sterile and non-toxic, and remove cell metabolites in time.
4.Constant cell growth temperature
To maintain the vigorous growth of cultured cells, it is necessary to have a constant and suitable temperature.
5.Suitable gas atmosphere
Gas is one of the necessary conditions for the survival of mammalian cell culture, and the main gases required are oxygen and carbon dioxide.
6.Cell culture temperature
To maintain the vigorous growth of cultured cells, it is necessary to have a constant and suitable temperature. Different types of cells have different requirements for culture temperatures. The standard temperature for human cell culture is 365℃±0.5. Deviation from this temperature range, the normal metabolism of cells will be affected, and even die.
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