1. Basic information of SLC3A2 CD98 recombinant protein:
SLC3A2 CD98 is a cell membrane protein encoding the gene SLC3A2 (Solute Carrier Family 3 Member 2), also known as CD98. SLC3A2 is a transmembrane protein composed of two subunits: one is the SLC3A2 subunit, which is the anchor subunit of the transmembrane; The other is the SLC7A5 subunit, which is located on the intracellular membrane surface. These two subunits are linked together by intercellular glycine binding contacts. The SLC3A2 CD98 protein is responsible for mediating the transport of amino acids between intracellular and extracellular. Catalog No. PA1000-5866
2. Experimental design:
This experiment was aimed at the function and application of SLC3A2 CD98 recombinant protein, and the following steps were adopted:
1.Obtain SLC3A2 CD98 recombinant protein:
a.Clone the SLC3A2 gene from human cells.
b.Transfer the SLC3A2 gene to a recombinant plasmid (e.g., PET-30A) and express the SLC3A2 CD98 protein using the E. coli BL21 (DE3) strain.
c.Expression and purity of recombinant proteins were confirmed by Western blot analysis, mass spectrometry identification, and SDS-PAGE assays.
2.Determination of the physiological properties of SLC3A2 CD98 protein:
a.The optimal temperature and pH value of the recombinant protein were determined, and its stability under different conditions was observed.
b.Nanoparticle tracking analysis (NTA) and other techniques were used to detect the particle size and distribution of recombinant proteins.
3.To study the amino acid transport function of SLC3A2 CD98 protein:
a.The SLC3A2 CD98 protein was conjugated to specific radiolabeled amino acids and the binding rate and efficiency were observed.
b.Fluorescence resonance energy transfer (FRET) experiments were used to verify the function of amino acid transport.
4.*Role of SLC3A2 CD98 protein in cell growth and transformation:
a.Transfect SLC3A2 CD98 protein into different cell types, and observe changes in cell proliferation, migration, and transformation ability.
b.Immunohistochemistry and other methods were used to study the expression and function of SLC3A2 CD98 in human tumors.
5.Applications for the identification of SLC3A2 CD98 protein:
a.A mouse xenograft model was used to study the effects of SLC3A2 CD98 protein on growth and metastasis.
b.In vivo and in vitro studies targeting SLC3A2 CD98 targeting ** for tumors, such as antibody-drug conjugation, etc.
3. Expected results of the experiment:
Through the above experimental design, it is expected that the expression purity and physiological characteristics of SLC3A2 CD98 recombinant protein can be obtained, so as to gain insight into its mechanism of action in biological processes such as amino acid transport, cell growth, migration and transformation. In addition, studying the expression and function of SLC3A2 CD98 in is helpful to explore its potential as a new ** target.
In summary, this article introduces the basic information of the SLC3A2 CD98 recombinant protein, and elaborates on the experimental design steps and expected results. Through the development of experiments, it is expected to further reveal the importance and application prospects of SLC3A2 CD98 protein in cell biology and **.