Tissue culture room purification project
1. Overview. The tissue culture room purification project is to provide a sterile, dust-free and harmless environment for plant tissue culture. Tissue culture is a technique for cultivating plant tissues or cells in vitro under laboratory conditions to achieve rapid plant propagation, variety improvement, genetic engineering and other applications. The degree of purification of the tissue culture room directly affects the success of the experiment, so the construction of the tissue culture room purification project is very important.
Second, the construction requirements of the purification project of the tissue culture room.
1.Air cleanliness requirements: The tissue culture room needs to achieve a certain air cleanliness to ensure that the room is sterile and dust-free. According to different experimental needs, different air cleanliness levels can be selected, such as ISO 14644-1 grade 1, grade 100, class 1000, etc.
2.Temperature and humidity requirements: The tissue culture room needs to maintain a constant temperature and humidity to maintain the normal growth of isolated tissues. Generally speaking, the temperature should be controlled at about 25 and the humidity should be controlled at about 70%.
3.Light requirements: Plant tissue culture requires certain light conditions to meet the needs of photosynthesis. The tissue culture room should be equipped with appropriate light equipment, such as LED lights, to ensure that the isolated tissues obtain appropriate light intensity and spectrum.
4.Ventilation requirements: In order to ensure the circulation and freshness of indoor air, the tissue culture room should have a good ventilation system to ensure the quality of indoor air.
5.Equipment requirements: The tissue culture room should be equipped with corresponding experimental equipment and instruments, such as ultra-clean workbenches, culture racks, incubators, microscopes, etc., to meet the needs of experiments.
6.Construction material requirements: In order to ensure the indoor air quality and environmental quality, the construction materials of the tissue culture room should be selected with antibacterial, dustproof, moisture-proof and other properties.
3. Key points of the construction of the purification project of the tissue culture room.
1.Pre-construction preparation: The construction site should be cleaned and disinfected before construction, and the site should be kept dry and level. At the same time, quality checks should be carried out on the building materials used to ensure that the quality of the materials meets the requirements.
2.Air purification system construction: According to the air cleanliness requirements, select appropriate air purification equipment and technology, such as high-efficiency filters, ultraviolet germicidal lamps, etc. At the same time, the position of the air supply outlet and the return air outlet should be reasonably arranged to ensure the quality of indoor air.
3.Construction of temperature and humidity control system: According to the experimental requirements, appropriate constant temperature and humidity equipment and technology, such as air conditioning system, humidifier, etc., should be selected. At the same time, temperature and humidity sensors and controllers should be reasonably arranged to ensure the stability of indoor temperature and humidity.
4.Lighting system construction: According to the experimental requirements, the appropriate lighting equipment and spectral distribution should be selected, such as LED lamps, fluorescent lamps, etc. At the same time, the position and number of lamps and lanterns should be reasonably arranged to ensure that the isolated tissues obtain suitable lighting conditions.
5.Ventilation system construction: According to the indoor air quality requirements, appropriate ventilation equipment and methods should be selected, such as fresh air system, exhaust system, etc. At the same time, the location of the vents and air ducts should be reasonably arranged to ensure the circulation of indoor air.
6.Laboratory layout design: The layout and process of the laboratory should be reasonably designed according to the experimental process and personnel flow to improve the experimental efficiency and management efficiency. At the same time, safety factors and environmental protection factors should be fully considered to ensure the safety and environmental performance of the laboratory.
7.Construction acceptance: After the construction is completed, the purification project should be fully accepted, including air cleanliness testing, temperature and humidity testing, light testing, ventilation testing, etc. For the links that do not meet the requirements, they should be adjusted and improved in time to ensure the construction quality and use effect of the tissue culture room purification project.