I. Introduction. The rabbit interhydroxy-epinephrine (MN) ELISA kit is a tool for the detection of MN levels in rabbit serum, plasma, or cell culture supernatants. MN is a catecholamine hormone that plays an important physiological role in the body. The kit has high sensitivity, specificity, and accuracy, providing a reliable detection method for scientific and clinical studies.
Second, the principle. The kit is based on the principle of enzyme-linked immunosorbent assay (ELISA), which detects the MN content in the sample through an antigen-antibody reaction. In the reaction, MN in the sample binds to the MN antibody coated in the wells of the polystyrene plate, and the enzyme-labeled MN antibody is added to form an immune complex. After washing, substrate is added for color development, and finally the absorbance value is measured by a microplate reader to calculate the MN concentration.
3. Composition of the kit.
The kit contains the following components:
1.Enzyme-labeled MN antibodies.
2.Polystyrene wells coated with MN antibody.
3.Chromogenic substrates.
4.Stop solution.
5.Standards.
6.Instructions.
Fourth, the operation steps.
1.Sample Handling: Rabbit serum, plasma, or cell culture supernatant should be diluted as appropriate.
2.Loading: Add the treated sample to a well of a polystyrene plate coated with MN antibody and incubate for 1 h37.
3.Plate washing: Wash the plate wells to remove unbound material.
4.Add enzyme-labeled antibody: Add enzyme-labeled MN antibody and incubate for 37 h for 1 h. Wash the plate again.
5.Chromogenic reaction: Add chromogenic substrate and incubate for 15-30 min37.
6.Stop the reaction: Add the stop solution to stop the color development reaction.
7.Detection: The absorbance value of each well was measured with a microplate reader and the data was recorded.
8.Calculation: A standard curve is plotted based on the standard concentration and absorbance value, and the concentration of mn in the sample is calculated from the curve.
5. Precautions.
1.Experiments are carried out in strict accordance with the operating procedures to ensure accuracy.
2.Kits should be stored at 2-8 °C to avoid repeated freeze-thaw cycles and prolonged exposure to sunlight.
3.Kits from batch to lot may vary and should be checked carefully before use.
4.Chromogenic substrates are susceptible to light exposure and should be stored away from light.
5.Do not use if the kit is abnormal or expired.
6.After use, dispose of the waste liquid in accordance with local regulations.