Recently, the Department of Pathology of Hunan Chest Hospital used cross-primer thermostatic amplification technology (CPA) to confirm the diagnosis of a complex case.
The man's symptoms did not resolve after surgery on his right knee, and he was diagnosed with tuberculosis
One year ago, Mr. Wan, who lives in Liuyang, began to have pain in his right knee joint, and after the removal of the lesion of the right knee joint in the local hospital, the pathology report showed that granulomatous inflammation was accompanied by necrosis, and the etiology showed that the smear and culture of Mycobacterium tuberculosis were negative. In order to seek further diagnosis and treatment, Mr. Wan went to Hunan Chest Hospital on the recommendation of the doctor.
After being admitted to the hospital, Zhang Shu, a technician from the pathology department of Hunan Provincial Chest Hospital, added the prepared reagents into the EP tube containing the wax roll of the patient's specimen in the biological safety cabinet, and then placed it into the easynat POCT tuberculosis rapid test system as required, and the specimen was shown to be positive one hour later. Combined with the results of pathological morphology and cross-primer constant temperature amplification (CPA), Mr. Wan was diagnosed with tuberculosis. Lei Zhanping, director of the Department of Pathology, immediately issued a diagnosis report, and immediately began to clinically effective anti-tuberculosis**.
Pathological diagnosis of tuberculosis is the key, and CPA detection is more accurate
Cross-primer isothermal amplification (CPA) was used to detect the specific IS6110 insertion sequence of Mycobacterium tuberculosis complex by fluorescent polymerase chain reaction. The reaction consists of 6 specific amplification primers, 1 specific fluorescent probe, and DNA polymerase with highly active strand displacement properties. Lei Zhanping introduced that under constant temperature conditions, the specific amplification process of the IS6110 fragment of Mycobacterium tuberculosis complex group was completed at one time, and the fluorescence signal was detected by the adaptive instrument and a real-time fluorescence curve was automatically generated, which could obtain accurate positive or negative evidence of tuberculosis.
Lei Zhanping said that in recent years, molecular pathology detection technology has developed rapidly, and CPA technology, as a molecular detection technology for the detection of Mycobacterium tuberculosis complex group DNA, has the advantages of simplicity, safety and efficiency, and can effectively improve the detection rate of Mycobacterium tuberculosis in paraffin tissue specimens. Pathological diagnosis is an important part of tuberculosis diagnosis, and accurate pathological diagnosis of tuberculosis can be made by combining molecular tests such as morphology and CPA. To provide favorable evidence for the early implementation of anti-tuberculosis** in patients with tuberculosis to achieve effective anti-tuberculosis** effect.
Manuscript**: Hunan Provincial Chest Hospital.
Author: Wang Xia Chen Siyu.