1. Experimental principle.
This kit uses an indirect enzyme-linked immunosorbent assay (ELISA) method to capture FBG in rabbit serum with rabbit anti-FBG antibody-coated microplates, followed by the addition of enzyme-labeled anti-FBG antibody, and finally the addition of substrate for color development. By measuring the absorbance value at 450 nm, the concentration of rabbit FBG in the sample can be measured indirectly.
2. Composition of the kit.
1.Microplate plates: Microplates coated with rabbit anti-FBG antibody.
2.Antibody: Enzyme-labeled anti-rabbit FBG antibody.
3.Standard: Rabbit FBG standard.
4.Wash solution: The wash solution used to wash microplates.
5.Substrate: A substrate solution used for color development.
6.Stop Solution: A stop solution used to stop the substrate reaction.
3. Steps.
1.Bring the kit and samples to room temperature and have all reagents ready.
2.In the appropriate position of the plate plate, add the standard and sample, respectively, and add 100 L wells, and then incubate at 37 for 1 h.
3.Wash the plate with washing solution for a total of 5 washes, each wash time is not less than 30 seconds.
4.Add 50 l wells of enzyme-labeled antibody and then incubate for 1 h at 37.
5.Wash the plate again with washing solution for a total of 5 times, each wash time is not less than 30 seconds.
6.The substrate solution was added and then incubated at 37 for 15-20 min.
7.The stop solution is added, and the absorbance value is then measured at 450 nm.
Fourth, the result calculation.
1.The absorbance value of the sample is converted to a concentration value by plotting a standard curve of the standard.
2.The concentration of rabbit FBG in the sample was calculated based on the dilution factor of the sample and the concentration of the standard.