Keywords: fluorescent quencher tide quencher 1 alkyne, TQ1 alkyne.
Product Introduction:
To do this, label the amino-modified oligonucleotides with Tide Quencher dye.
1.Prepare Oligo solution (Solution A) dissolve amino-modified Oligo (200 g) in tetraborate buffer (100 L, pH 8.).5±0.5) Medium.
2.Prepare the dye solution (solution B).
2.Dissolve 1 mg of dye SE in 100 LDMSO (> 10 mg ml if possible) by pipetting up and down. Centrifuge the solution stock solution from the side of the vial to the bottom of the vial.
Note: Prepare the DMSO dye solution before starting conjugation. Long-term storage of the dye solution can reduce dye activity. Any solution containing the dye should be protected from light. We do not recommend that you store the DMSO dye solution for future use.
3.Run the conjugation reaction.
3.1 Add the oligomer solution (A, 100 L) to the dye solution (B, 20-50 L) while stirring or shaking (keeping the reaction mixture protected from light).
3.2. Rotate or shake the reaction mixture on a rotator or shaker for 4-6 h at room temperature.
Note: Gently vortex the vial every 10 min during the first hour to ensure that the reaction solution remains well mixed. Do not mix vigorously as the material may remain on both sides of the vial. After six hours, 50-90 amine-modified oligonucleotide molecules should be labeled. If more convenient, the reaction can be incubated overnight. However, in most cases, overnight incubation does not result in higher labeling efficiency.
4.Purify dyes oligosaccharide conjugates.
4.1 Initial purification was performed by ethanol precipitation of labeled oligonucleotides.
4.2 Final purification by HPLC or gel electrophoresis.
Related parameters:
Chinese name: fluorescent quencher tide quencher 1 alkyne, tq1 alkyne.
English name: tide quencher 1 alkyne,tq1 alkyne
Molecular weight: 32740
Appearance: Solid powder.
Specifications: 1g, 5g, 10g
Purity: 95%.
Storage Recommendation: Store at -20°C, away from light and moisture. It must be dried to avoid repeated freezing and thawing.
Precautions: In places where dust is generated, provide suitable exhaust equipment. Do not allow the product to get into the drain. This product is for scientific research purposes only and is not intended as a drug, home backup medicine or other use.
Related reagents of the same type:
Fluorescent quencher tide quencher 1 maleimide.
Fluorescent quencher tide quencher 1 phosphoramidite.
Fluorescent quencher tide quencher 1 succinimidyl ester.
Fluorescent quencher tide quencher 2 acid.
Fluorescent quencher 2 amine.
Fluorescent quencher tide quencher 3ws acid.
Fluorescent quencher tide quencher 3 azide.
Fluorescent quencher tide quencher 3 alkynes.
Fluorescent quencher 2ws alkynes.
Fluorescent quencher Tide Quencher 4WS-DBCO [TQ4WS-DBCO].
Fluorescent quencher tide quencher 7ws azide.
Fluorescent quencher tide quencher 51ws acid [tq5.1ws acid]
Fluorescent quencher tide quencher 51ws amine [tq5.1ws amine]
Fluorescent quencher tide quencher 71ws maleimide [TQ7.]1ws maleimide].
Fluorescent quencher tide quencher 71ws succinimidyl ester [TQ7.]1ws se]
Fluorescent quencher tide quencher 71ws alkyne [tq7.]1ws alkynes].
Fluorescent quencher tide quencher 72ws acid [TQ7.]2ws acid].
Fluorescent quencher tide quencher 72ws maleimide [TQ7.]2ws maleimide].
Fluorescent quencher tide quencher 72ws azide [TQ7.]2ws azide].
The above information is compiled by Shaanxi Xinyan Bomei Biotechnology Mumu