Fluorescence quencher 2 phosphoramidite is a highly sensitive fluorescent quencher that can be used in various fluorescence analysis techniques, such as fluorescence immunoassays, fluorescent probe technology, etc. The substance emits strong fluorescence when excited by ultraviolet light, but it does not participate in the quenching process of the fluorescence reaction itself. In contrast, Tide Quencher 2 phosphoramidites can quench fluorescence by capturing energy or adsorbents to eliminate fluorescence emitted by other fluorescent species.
In fluorescent immunoassays, Tide Quencher 2 phosphoramidites can be used to label antibodies or antigens to enhance the detection of fluorescent signals. By conjugating Tide Quencher 2 phosphoramidites to an antibody or antigen, the intensity and stability of the fluorescent signal can be increased, thereby improving the sensitivity and specificity of the assay. In addition, Tide Quencher 2 phosphoramidites can also be used in fluorescent probe techniques such as fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging (FLIM). Among these techniques, Tide Quencher 2 phosphoramidites can be used as an energy transfer agent or quencherizer to achieve precise detection and localization of specific fluorescent species.
In conclusion, the fluorescent quencher 2 phosphoramidite is an important fluorescence analysis tool, which has a wide range of application prospects in the fields of fluorescence immunoassay and fluorescent probe technology. The sensitivity and specificity of fluorescence analysis can be greatly improved through the rational selection and use of Tide Quencher 2 phosphoramidite.
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