Chinese name: dota-tri-tert-butyl-succinimidyl ester.
Chinese aliases: dota-tri-tert-butyl ester-active ester.
Product Name: dota-(coot-bu)3-nhs
Chemical Name: 1,4,7,10-Tetraazacyclopentadecane-1,4,7,10-tetraacetic acid-(coot-bu)3-reactive ester.
CAS Number: 819869-77-7
MF: C32H55N5O10
Molecular weight: 66981
Storage conditions: -20, dry, avoid frequent thawing and freezing.
Solubility: soluble in most organic solvents, soluble in water.
Dota-(Coot-Bu)3-NHS is a commonly used polypeptide linker, and its chemical name is 1,4,7,10-tetraazacyclopentadecane-1,4,7,10-tetraacetic acid-(coot-bu)3-reactive ester. It is a highly effective peptide coupling agent used to conjugate peptides to antibodies, proteins, or other biomolecules.
In peptide synthesis, linkers are a key component that is able to link one peptide to another peptide or a solid support. The DOTA-(coot-bu)3-NHS linker offers a number of advantages, including high stability, good water solubility, and low immunogenicity. These properties make it a very useful tool for the preparation of biologically active peptide and protein complexes.
In peptide synthesis, peptides are usually synthesized using solid supports. There are several ways to synthesize peptides on solid supports, the most common of which is the use of an FMOC TBU guard system. During synthesis, each amino acid residue is selectively linked to a solid support, and then the protecting group is removed at each step so that the next amino acid residue can be added in the next round of synthesis. Finally, the synthesized peptide can be cleaved from the solid support by using a linker and then subjected to further processing or modification.