The basic principle of centrifuge tubes, how to use and precautions
Centrifuge tubes should not exceed their volume is an important principle in centrifugation operations, if there is too much liquid in the tube, or if the density and centrifugation conditions of the liquid are not appropriate, it will cause the tube to rupture, resulting in the loss of sample. This problem is easy to ignore and should be paid special attention to in experiments.
The selection of centrifuge tubes should be based on specific experimental needs, such as the amount of sample to be tested, the time and speed of centrifugation, etc. Centrifuge tubes are generally divided into two types: ordinary centrifuge tubes and low-temperature centrifuge tubes, which should be paid attention to when selecting.
The method of centrifuge the tube is as follows.
1.Choose the right centrifuge tubeCentrifuge tubes are divided into different capacities and materials, and choose the appropriate centrifuge tubes according to the needs of the experiment. Commonly used tubes have a capacity of 05ml、1.5ml and 2ml, etc.
2.Prepare the sampleAccording to the experimental needs, pour the liquid sample to be tested into the centrifuge tube. Be careful not to exceed the reticle of the centrifuge tube to avoid sample spillage.
3.Closed tubesAfter pouring the sample into the tube, seal the tube with a matching cap. Make sure the cap of the centrifuge tube is tightly closed to prevent leakage during centrifugation.
4.Set centrifugation parametersAccording to the needs of the experiment, set the speed and time of the centrifuge. Higher rotational speeds and longer centrifugation times are typically used to separate heavier components, while smaller rotational speeds and short times are used to separate lighter components.
5.Perform centrifugation Place the sealed centrifuge tube into the centrifuge, making sure the centrifuge lid is closed and securely locked. Start the centrifuge and let the centrifuge run for the time it takes.
6.When the stop centrifugation time is over, stop the centrifuge and carefully remove the centrifuge tube. Be careful not to invert or shake the centrifuge tube to avoid mixing the separated ingredients.
7.ObservationsObserve the sample in the tube, where the heavy components are deposited at the bottom of the tube and the light components float on it. Both ingredients can be transferred separately to a new container for further analysis and research as needed.
All in all, centrifuge tubes are a simple yet effective experimental tool that is widely used in fields such as biochemistry, molecular biology, medicine, and environmental science. The correct use of centrifuge tubes can improve the accuracy and reproducibility of experiments and provide strong support for scientific research.
Precautions for the use of centrifuge tubes:
1.The centrifugation speed should be gradually increased, and the centrifuge door should be opened only after the centrifuge tube has completely stopped.
2.When using centrifuge tubes, pay attention to the bottom of the centrifuge tube with the fixed adapter on the centrifuge to ensure safety.
3.When sampling, avoid reusing the same centrifuge tube to avoid interfering with the assay results.
4.The carrying range of the tube, i.e. the capacity of the sample must not exceed the limit of the tube, otherwise it will cause damage to the tube. In particular, the centrifuge tube should not exceed its volume.
5.Centrifuge tubes should not be used for too long, generally not more than 12 months.