[Evaluation principle].
The half-lethal concentration (LC50) refers to the toxic concentration that causes half of the tested animals to die in an acute toxicity test in animals. LC50 is used as a basis for studies comparing the toxicity of various pollutants, the susceptibility of animals of different species or stages of development to pollutants, and the effects of environmental factors on toxicity. Routine acute toxicity experiments are often carried out in rodents and non-rodents, which can more accurately reflect the safety of drugs, but the experimental cycle is generally longer and the dosage of drugs is larger. It is an ideal test model to determine the toxic effect of drug exposure in embryos and evaluate the lethal concentration of half of the drug by using zebrafish embryos to detect the acute toxicity of drugs, which has many advantages such as small size, low cost, easy to feed, good repeatability, easy to observe, short reproductive cycle, high reproductive frequency, and simple administration method.
Zebrafish mortality according to the experimental endpoint by using GraphPad Prism 80.1 Statistical software, fitting half lethal concentrations.
On the way home is the annual zebrafish "concentration-mortality" effect curve.
References] Yuan Wenlin, Huang Zhengrui, Xiao Sijia, et al. Study on the acute toxicity of flavonoids from the root of mountain bean to zebrafish[J].Chinese Herbal Medicine,2021,52(10):2978-2986