Product name: Fluorescent dye Cy5 labeled enrofloxacin.
Product Name: Cy5-enrofloxacin
*Business: Chongqing Xinweichuang.
The basic principle of fluorescently labeled enrofloxacin technology is to label enrofloxacin molecules with a specific fluorescent substance so that they emit a fluorescent signal in response to excitation light. The intensity of the fluorescent signal is directly proportional to the enrofloxacin concentration, so the concentration of enrofloxacin can be detected by measuring the intensity of the fluorescent signal. This method has the advantages of high sensitivity, high specificity, and rapid detection.
The implementation steps of fluorescently labeled enrofloxacin technology mainly include the selection of fluorescent substances, the optimization of labeling conditions, the drawing of standard curves, and the detection of actual samples. Among them, the selection of fluorescent substances is one of the key links. Commonly used fluorescent substances include fluorescent dyes, quantum dots, fluorescent proteins, etc. In the process of labeling, it is necessary to select appropriate fluorescent substances and labeling conditions to ensure that fluorescently labeled enrofloxacin has good stability and biological activity.
The drawing of the standard curve is another key part of the fluorescently labeled enrofloxacin technique. By fluorescently labeling and measuring different concentrations of enrofloxacin, a curve of the fluorescence signal as a function of enrofloxacin concentration can be obtained. The accuracy of the standard curve has a direct impact on the test results of the actual sample. Therefore, it is necessary to ensure the consistency and reproducibility of experimental conditions when plotting the standard curve.
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