It is required to be able to provide general and specific biological traits of cells. The general characteristic indicators include: general morphology of cells, specific structure, cell growth curve and ** index, doubling time, inoculation rate, chromosome analysis, isozyme examination, DNA fingerprint, etc. Specificity is often identified based on the specificity of the cell type and function, for example, in the case of glandular cells, the presence of specific products, including secreted proteins or hormonesIf it is a tumor cell, it should also be able to prove that the cell is indeed the first to the tumor tissue and not others, and still retains the characteristics of the tumor tissue, for which colony formation experiments, nude mouse tumorigenicity experiments and invasion experiments on normal tissues are often done.
1. Identification of normal cell lines
The identification of normal cell lines is mainly carried out around the following four aspects:
1) Identification of cell germline**: Commonly used methods mainly include chromosome analysis, isozyme analysis, DNA fingerprinting and other techniques.
2) Identification of tissue** of cells: Identification can be carried out by morphological means, detection of tissue-specific antigens, etc.
3) Whether the cells undergo transformation and malignant transformation: It is mainly identified by karyotype analysis, observation of cell growth behavior (whether contact inhibition is lost), and tumorigenesis experiments in nude mice.
4) Whether there is cross-contamination in cells, mainly identified by isozyme and DNA fingerprinting technology.
2. Identification of tumor cell lines
The identification of tumor cell lines mainly revolves around their malignancy, chromosomal abnormalities, changes in contact inhibition and density-dependent growth characteristics, colony formation ability, tumorigenesis in nude mice, invasive growth in animals, and some characteristics at the gene and molecular levels are the directions of tumor cell identification.
Significance of STR identification of cells:
Due to the importance of cell culture systems in the research and technology development of biopharmaceuticals, the proper cell line identification process is a major interest for every researcher. However, the problem of cross-contamination remains. As the number and frequency of new cell lines used in laboratories around the world increases, there are significant differences in the basic principles of quality control (e.g., cell line identification). From what has already been published、Use"Wrong"From cell lines leading to questionable results in research**, to stem cell lines and other cell lines for clinical use, cross-contamination affects every area of scientific research – from benchtop to clinical. Without significant changes in the handling and handling of cell cultures, cross-contamination will become a much bigger and more serious problem.
Cell culture systems are very important in the development of biological research and drug research, and with the deepening of research, the types of cell lines are gradually increasing, and the cross-contamination of cell lines still exists. Cross-contamination is a serious problem for experimental studies, and if the results of the study are questioned due to the use of the wrong cell line, previous experiments may have to be redone. Therefore, the certification of the cell line is necessary.