Product name: Fluorescently labeled polyglutamic acid.
*Business: Chongqing Xinweichuang.
Product name: Fluorescein FITC conjugated polyglutamic acid.
Product Name:fitc-pga
*Business: Chongqing Xinweichuang.
1.Target labeling: Select the appropriate dye to label the target cells or tissues according to your research needs. Labeling can be done by direct injection of dye solutions, gene transfection, or viral vector transduction.
2.Animal preparation: Ensure that the animal is in good health and given appropriate anesthesia to reduce stress and motion artifacts before performing imaging.
3.Imaging equipment selection: According to the location and depth of the target organ or tissue, select the appropriate fluorescence microscope or related equipment for imaging. At the same time, ensure the compatibility of the excitation and emission wavelengths of the device with the selected dye.
4.Imaging parameter setting: According to the type of device and dye characteristics, set the appropriate imaging parameters, such as ** time, filter combination, scanning speed, etc.
5.Data collection and analysis: Collect image data through equipment, and use related software for data analysis, processing, and visualization. Dynamic changes such as distribution, proliferation, and migration of target cells can be observed.
6.Post-experiment treatment: After the experiment, the small animals are handled appropriately to reduce unnecessary injury and suffering. For example, administering an appropriate anesthetic for euthanasia or removing the target organ for further analysis.
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Fluorescent proteins