Nanoscale CD8 sorting magnetic beads can be used to sort human CD8+ T cells, and CD8+ T cells can be sorted by conjugating an anti-human CD8 monoclonal antibody to the beads. Nanoscale CD8 sorting magnetic beads were incubated with PBMCs and magnetically separated, so that CD8+ T cells were isolated and enriched, and played the role and function of purifying CD8+ T cells. Suitable for the production of cell** products.
Product Information
How to use:
1.Resuspend human PBMC cells in PBS buffer containing 1% HSA, sample count, and take 1x107 cells to 1In a 5 ml EP tube, 300 g, centrifuge for 5 min.
2.Discard the supernatant, resuspend 80 L of PBS buffer solution containing 1% HSA, add 20 LCD 8 sorting magnetic beads, mix well, and incubate in a 2 8 freezer for 15 min.
3.Place the XL sorting column onto a sorter and rinse twice with 1 ml of PBS buffer containing 1% HSA.
4.Remove the incubated samples from the 2 8C freezer, add 1 ml of PBS buffer with 1% HSA, 300 g, centrifuge for 5 min, and discard the supernatant.
5.Add 1 ml of PBS buffer with 1% HSA to resuspend, add the sample to the sorting column, allow it to flow out naturally, add PBS buffer with 1% HSA in two parts, add 1 ml each time, and collect the flow-through in a 15 ml centrifuge tube.
6.After all the PBS buffer containing 1% HSA has flowed out, remove the sorting column from the sorter, put it into a new 15 ml centrifuge tube, add 3 ml of PBS buffer containing 1% HSA to the sorting column, and use the piston of the XL sorting column to directly punch the liquid.
Storage conditions
2-8 Store in the dark, do not freeze. The expiration date is shown on the outer label of the reagent.
Related Products
This product is even more effective with our SOPHMAG manual magnetic sorting kit.