Research ELISAThere are many names in China, such as: ELISA detection kit, ELISA, enzyme-linked immunosorbent assay kit, enzyme-linked immunosorbent assay kit, enzyme-linked immunoassay kit, enzyme-immunoassay kit, etc., the more common names are ELISA detection kit, enzyme-linked immunosorbent assay kit, etc. Optimization of ELISA kit dispensing: In this process, there are generally three dosings, which are specimens, enzymes, and substrates. After thawing the frozen serum, the protein part is concentrated and unevenly distributed, so it should be fully mixed gently to prevent bubbles, and it can be mixed upside down and down, and do not vibrate violently on the mixer. Anticoagulants are used to prepare plasma specimens, while serum specimens can be obtained only after natural agglutination and shortening of serum. Dilutions can also be fed in the plate wells, followed by the serum specimens in which they are fed, and then vibrated on a microvibrator for 1 min to ensure mixing.
In general, serum specimens measured within 5 days can be placed at 4 °C, and those measured over a week should be stored on low temperature ice. 1. Serum: Collected with a sterile tube, the blood will naturally coagulate for 10-20 minutes at room temperature, centrifuge for about 20 minutes (2000-3000 rpm) under 2-8 conditions, carefully collect the supernatant, and centrifuge again if there is accumulation during storage. 2. Urine: Collected with a sterile tube, centrifuged for about 20 minutes (2000-3000 rpm) under 2-8 conditions. Carefully collect the supernatant, and if there is any accumulation during the preservation process, it should be centrifuged again. 3. Plasma: EDTA, heparin sodium or sodium citrate should be selected as anticoagulants according to the requirements of the specimen, mixed for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm) under 2-8 conditions, carefully collected and supernatant, and centrifuged again if there is accumulation during the preservation process. 4. Chest and ascites fluid: Collect with a sterile tube, centrifuge for about 20 minutes (2000-3000 rpm) under 2-8 conditions, carefully collect the supernatant, and centrifuge again if there is accumulation during the storage process.
5. Cerebrospinal fluid: Collect with sterile tubes, centrifuge for about 20 minutes (2000-3000 rpm) under 2-8 conditions, carefully collect the supernatant, and centrifuge again if there is accumulation during the preservation process. 6. Saliva: Collected with a sterile tube, centrifuged for about 20 minutes (2000-3000 rpm) under 2-8 conditions, carefully collected and supernatant, and centrifuged again if there is accumulation during the preservation process. 7. Cell culture supernatant: When detecting secretory components, collect them with a sterile tube. 2-8 Centrifuge for about 20 minutes (2000-3000 rpm), carefully collect the supernatant, and centrifuge again if there is any accumulation during the storage process. 8. Milk: Collect with sterile tubes, centrifuge for about 20 minutes (2000-3000 rpm) under 2-8 conditions, carefully collect the supernatant, and centrifuge again if there is accumulation during the storage process.