Since microbial testing covers almost all the products of food enterprises, microbiology is one of the internal environments of enterprises.
The object room is the hotbed of the product and the cornerstone of the accuracy of the microbial experiment. Therefore, establish a qualified microbiology room and schedule it
Regular monitoring of the environment is a guarantee of the reliability of the experiment. This article examines the effects of the microbiology laboratory environment on experiments.
What conditions should be met in the room, and how to carry out environmental monitoring are discussed.
1 Architecturally: the microbiology laboratory is divided into a sterile operation room and a buffer room, and the experimenters should go to the dressing room first, and then go to the bathroom.
Punch the room, and finally enter the sterile room. The work area of the laboratory is clearly separated from the office area. The aseptic operation room should be responsive to the cleanliness level.
In other operating environments, when local 100-level measures are adopted, the environment should meet the requirements of 10,000-level cleanliness, and the diameter of each cubic meter is greater than or equal to 05
The number of dust particles in microns should be less than or equal to 350,000, and the number of dust colonies in 30 minutes should be less than or equal to 3cfu cubic meters. If empty.
If the air cleanliness does not meet this requirement, it is recommended to set up an ultra-clean workbench. Entering the sterile room should be a clean and clean facility; Aseptic.
According to the needs of the inspected varieties, the relative positive pressure or relative negative pressure on the adjacent chamber should be maintained, and the cleanliness should be tested regularly. If yes do.
Pharmaceutical enterprises, the daily clean area of 10,000 and 100 areas of the plankton bacteria should also be tested once a month, which is the national standard of plankton detection.
method as stated above. The first thing to test cleanliness is to monitor the disinfection effect of the sterile room: nutrient agar plates with a diameter of 9 cm.
For air exposure sampling, 3 points (diagonal) can be taken, exposed for 5 minutes, and then incubated for 36 hours for 24 hours to observe the number of colonies. Press.
The result of the column formula is as follows: total number of bacterial colonies in the air (CFU m3) = 5000n a*t, a: plate area (square centimeters) t: average exposure time.
min), n: average colony cfu flat dish. The disinfection effect of ordinary clean rooms should be monitored at least once a month. Secondly, after disinfection, right.
Sterility experiment for air quality monitoring: put an ordinary nutrient agar dish with a diameter of 9cm in the four corners of the sterile room and a piece of ** (set.
A blank control), open the lid of the flat dish and put it next to the flat dish, and leave it open for 10 minutes to cover. Plate the plates to 37 for 24 h and observe: Calculate the average number of colonies in the 5 plates.
2. From the perspective of instruments and equipment: the experimental equipment should meet the needs of the inspection work. Microbiology laboratories must be equipped with incubators, high pressure.
Sterilizers, UV lamps, refrigerators and other equipment. Here we focus on UV lamps and autoclaves. The ultraviolet lamp is the main microbiology room.
The penetration ability of the UV lamp is particularly weak, so the UV lamp must be cleaned regularly and regularly. In general, every.
Use a rag soaked in 75% ethanol to wipe the lamp once a month, unplug it and cool it for 10 minutes before wiping, and the irradiation intensity of the ultraviolet lamp should be tested frequently, when the irradiation intensity is lower than 70% of the original irradiation intensity, the lamp should be replaced by a professional, and the irradiation intensity of the ultraviolet lamp should be checked every three months under normal circumstances, and the monitoring results should be recorded (
For the disinfection of test tubes, straws, nutrient agar plates and other utensils commonly used in the microbiology laboratory, the most commonly used sterilizer in the laboratory is the hand.
Lift autoclave. It works by increasing the pressure in the pot and increasing the temperature to increase the vapor temperature.
And to achieve the purpose of complete sterilization. The following methods can be used for monitoring portable pressure cookers: number of samples: 3 pieces, step: about to be born.
The material indicator is placed in the center of the item and one on the outer layer of the item to be disinfected, and it is disinfected according to routine. Sterilize the biological indicators with sterile hands.
Continued to add bromocresol purple glucose peptone water and incubate in a 55-60 incubator for 7 days to observe the final result. If there is a thermophilic fat rod.
The bacteria grow, the medium changes from purple to yellow, and there is a piece of bacteria inside and outside the package, which is regarded as a high-pressure cooker sterilization and is not qualified.
3. In terms of location and structural layout: the working area and overall layout of the laboratory should be able to meet the needs of inspection work and experiments.
The room layout should be a one-way workflow. The location of the microbiology laboratory should be slightly farther away from the workshop, boiler room, and traffic arteries to reduce the infestation of dust from chimneys and roads. The structure of the house should be shockproof, fireproof, dustproof, and well-lit. Houses should be built.
The suites are separated from each other. The antibiotic microbiology laboratory should be divided into a semi-aseptic operation room and a buffer room, the semi-aseptic operation room should be equipped with ultraviolet lamps, the operating table should be stable and kept horizontal, the laboratory should be brightly lit, equipped with equipment to control temperature and relative humidity, and cross-infection should be noted. The ambient temperature and humidity of the microbiological laboratory also need to be monitored, and the general recommended temperature is about 20 and the humidity is about 50-60.
4. From the perspective of the internal environment of the laboratory: the walls, ceilings and floors of the laboratory should be flat, easy to clean, impermeable and chemical-resistant.
corrosion of products and disinfectants. The floor should be non-slip and no carpet should be laid. Each laboratory has a floor drain to reduce the risk of water accidents.
The laboratory floor should be wet mopping, and dry mopping and dry sweeping are prohibited. When disinfection is required, it can be sprayed with a chlorine disinfectant with an effective chlorine of 500-10000mg l.
or mopping the floor, the amount of disinfectant is not less than 100ml cubic meters. Mops in polluted areas and clean areas should be used separately and should not be mixed. After use, use.
Soak the disinfectant solution for 30 minutes, then wash it with water and hang it to dry.
In short, since environmental conditions include both physical, chemical, and biological factors, adverse environmental conditions can cause microscopic factors.
The growth of organisms is inhibited, which even leads to the death of the thallus. But some microorganisms produce spores that are resistant to harsh environmental conditions.
Strong resistance. When different species of microorganisms appear in a limited space, they become environments and influence each other.
Echoes are both interdependent and mutually exclusive, showing a complex relationship between them, and this complexity seriously affects the reality of microorganisms.
The test results affect the authenticity and reliability of the data. Therefore, it is important to control the environment of the microbiology laboratory and monitor it regularly.
The first consideration and implementation of the problem of industry and quality inspection personnel. This is not only a requirement of the experiment, but also a manifestation of responsibility to enterprises and consumers.