Product name: Cyanine dye Cy5 carboxyl group.
Product alias: cyanine dye cy5 carboxylic acid.
Product Name: cy5-cooh
CAS Number: 1032678-07-1
Cy5-Cooh fluorescent dyes are mainly used for biomarkers and imaging, and the following are how to use Cy5-Cooh fluorescent dyes:
Dye dissolution: Cy5-COOH is usually in powder form**. Before use, it needs to be dissolved in an appropriate solvent such as dimethyl sulfoxide (DMSO) or methanol.
Dye concentration: Determine the appropriate concentration of Cy5-CoOH for your specific application. In general, the concentration of the dye should be at 001 to 0between 1 mmol.
Covalent conjugation: If you plan to use Cy5-CoOH to label other molecules, such as antibodies, proteins, or nucleic acids, covalent conjugation is required. This typically involves reacting the dye with an amino group or other suitable functional group in the target molecule.
Reaction time and temperature: In a covalent coupling reaction, the reaction time and temperature need to be controlled to ensure that the reaction proceeds adequately.
The dye should be stored in a cool, dry, and dark place to prevent photodegradation.
Fluorescent dyes Related material recommendations:
Fat-soluble -Cy5-carboxyl Cy5-Cooh 1032678-07-1
Water-soluble Cy5-carboxyl sulfo-cy5-cooh 146368-11-8
Fat-soluble-Cy5-aminoCy5-NH2 1807529-70-9
Water-soluble-cy5-aminosulfo-cy5-nh2 2183440-44-8
Fat-soluble -Cy5-active ester Cy5-NHS 146368-14-1
Water-soluble -cy5-reactive ester sulfo-cy5-nhs 2230212-27-6
Fat-soluble -cy5-maleimide Cy5-MAL 1437796-65-0