The non-histone chromosomal protein HMG-14, also known as nucleosome-bound domain 1 of the high-mobility group, is a protein encoded by the HMGN1 gene. The chromosomal protein HMG14 and its analogue HMG17 (MIM 163910) bind to the inner side of ribosomal DNA, potentially altering the interaction between DNA and histone octamers. These 2 proteins may be involved in maintaining transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the highly evolutionarily conserved DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in important cellular functions. HMGN1 has been shown to interact with YWHAZ.
The high-mobility group nucleosome-binding domain protein 1 (HMGn1) is a non-histone chromosomal protein that is a member of the high-mobility family (HMG) protein superfamily. HMG proteins are a class of proteins that bind to DNA and have high mobility, and they play an important role in chromatin structure and gene expression regulation.
HMGN1 has a unique structural feature that contains one or more HMG cassettes, which are the main regions where it binds to DNA. The HMG cassette is a sequence rich in amino acid residues that binds to the small groove region in the double helix structure of DNA. This binding capacity allows HMGN1 to move through chromatin and interact with specific DNA sequences.
The main function of HMGN1 is to regulate the structure and dynamics of chromatin. It can interact with histones and other non-histone proteins, influencing the compression and unfolding state of chromatin. In addition, HMGN1 can also bind to specific DNA sequences to regulate gene expression. Studies have shown that HMGN1 plays an important role in cell differentiation, proliferation, and apoptosis.
In addition to HMGN1, the high-mobility group protein superfamily includes other members, such as HMG14 and HMG17. These proteins also have the ability to bind to DNA and play an important regulatory role in different life processes.
In conclusion, nucleosome-binding domain protein 1 (HMGN1) is an important chromatin-binding protein that is involved in many important life activities by regulating chromatin structure and gene expression regulation.
The detection methods of high-mobility nucleosome binding domain protein 1 (HMGN1) mainly include western blot, immunoprecipitation and real-time quantitative PCR.