**Recommended
Protective effect of coriander root extract on skeletal muscle homeostasis in long-term exercise-induced fatigue mice
Author:
Wang Cheng, Yu Zhongru, Li Anyi, Zhu Hongkang, Guo Yahui, Cheng Yuliang, Qian He*
Unit:
College of Food Science, Jiangnan University.
**Item:
National Key R&D Program of China (2022YFF1101103).
Abstract > Keywords
Summary:Coriander root is a traditional crop with the same origin as medicine and food, which mainly grows on the plateau and has anti-hypoxia and anti-fatigue effects. In order to explore the functional activity and protective effect of coriander root extract on muscle homeostasis, its antioxidant activity was first determined by in vitro free radical scavenging experiments. Long-term exercise-induced fatigue was used in mouse models, and blank group, exercise group, low and high dose groups were set up respectively. The protective effect against muscle injury was determined by histomorphological observations. Then, using biochemical index detection and real-time quantitative PCR experiments, it was found that the extract of coriander root increased the ratio of NAD+ NADH and promoted the synthesis of ATP. Mitochondrial function is improved by modulating the SIRT1 PGC-1 TFAM pathway. By regulating the NRF2 HO-1 pathway, the antioxidant stress defense system of mice was effectively improved. Finally, by regulating the expression of actin and titin-related genes, muscle regeneration was promoted and muscle damage was alleviated. In summary, coriander root can enhance energy metabolism, maintain mitochondrial function stability, improve antioxidant defense, alleviate muscle damage and promote muscle regeneration, thereby playing a role in protecting muscle homeostasis.
Keywords:Coriander root;Exercise fatigue;Mitochondrial function;Muscle homeostasis.
Key conclusions:
1.Antioxidant activity of coriander root:
Coriander root extract (AEB) showed good antioxidant activity in different free radical scavenging experiments, and the half inhibition rates of DPPH, ABTS cationic free radical and OH were respectively. 473 3 mg/ml。This suggests that coriander root has potential antioxidant capacity and may help reduce damage caused by free radicals in the body.
A-DPPH free radical scavenging activity;B-ABTS cationic radical scavenging activity;Scavenging activity of C-OH;D-iron ion reducing force Figure 1 AEB antioxidant activity in vitro Fig1 antioxidant activity of aeb in vitro2.Morphological observation of muscle tissue:
AEB has a protective effect against exercise-induced muscle damage, reducing muscle cracks, necrosis, and swelling. AEB intake can enhance muscle fiber diameter and density, improve muscle strength, and alleviate exercise-induced muscle homeostasis.
a-Muscle slices;b-myofiber diameter;c-myofiber density;D-Leukocyte infiltration number (N3) Figure 2 Effect on muscle tissue morphology2 effects on histological changes in muscleNote: *p < 005、**p < 0.01、**p < 0.001 vs.con group. #p < 0.05、##p < 0.01、###p < 0.001 vs.EX group, the same below. 3.Muscle Energy Metabolism Effects:
AEB intervention helps to increase muscle ATP content, regulate NAD+ NADH ratio, and promote glycolysis and TCA circulation, thereby meeting the energy needs of muscles during strenuous exercise.
4.Muscle mitochondrial function affects:
AEB may regulate mitochondrial biogenesis through the SIRT1 PGC-1 TFAM pathway, enhance muscle mitochondrial function, and thereby ameliorate exercise-induced mitochondrial dysfunction.
5.Muscle Antioxidant Capacity Effects:
AEB intake can reduce muscle peroxidation status and increase antioxidant enzyme activity caused by strenuous exercise, possibly by enhancing the NRF2 pathway.
6.Muscle regeneration-related genes affect:
AEB treatment helps to promote myofiber repair and remodeling, promotes muscle regeneration by regulating the expression of actin and titin-related genes, and alleviates muscle damage caused by strenuous exercise.
In summary, studies have shown that coriander root extract (AEB) has significant multiple effects such as antioxidant, muscle tissue protection, regulation of energy metabolism and mitochondrial function, improvement of muscle antioxidant capacity, and promotion of muscle regeneration, which has a potential positive impact on alleviating exercise-induced muscle damage.
Meet the team. 
Team Profile:
1 professor (doctoral supervisor): Qian and teacher.
2 associate professors: Cheng Yuliang, Guo Yahui, 1 postdoctor;There are 3 doctoral students and 21 master's students.
Research Interests:
Food quality and safety control and management;Research and development of new food raw materials and functional foods;Rapid detection of foodborne contaminants (nanotechnology);Current research points: research and development of health food, such as innovative research and development of various soy products, functional research of red yeast and its oil, research on the industrialization of medicinal and edible homologous food, food quality and safety control and management, etc.
Team Results:
He has published more than 10 monographs, including Food Quality Control and Management, Food Safety Control and Management, Food Hygiene: Principles and Practice, and Food Safety Laws, Regulations and Standards.
Publications: Published more than 100 articles, including critical reviews in food science and nutrition, free radical biology and medicine, nanoscale, food and agricultural immunology, journal of agricultural and food chemistry and other authoritative journals at home and abroad.
Research projects: presided over and completed more than 20 scientific research projects such as the National Key R&D Program, the "12th Five-Year Plan" and the "13th Five-Year Plan" of the Ministry of Science and Technology
Team Culture:
The team atmosphere is positive, encouraging and supporting students' innovative thinking, and focusing on the all-round development of studentsStudents have strong comprehensive ability, and most of the graduates are working in the world's top 500 companies, key food companies at home and abroad, etc
Corresponding author
Qian He is a professor and doctoral supervisor at the School of Food Science, Jiangnan University. Winner of the title of "Advanced Individual in National Textbook Construction". He used to be a senior auditor of HACCP, an ISO9001 auditor, and a training teacher for food safety management system auditors of China Quality Certification Group (CQC).Expert of Wuxi Food Standard Professional Technical Committee, Expert of Jiangsu Provincial Food Safety Standard Evaluation Committee;Vice Chairman of the Free Radical Preventive Medicine Professional Committee of the Chinese Preventive Medicine Association. Wide range of interests;I like to read and think;Optimistic personality and humble to others.
This article is based on the original article and the author's team.