I. Introduction. Ethylene is an important hormone in plant growth regulators, which plays an important role in the growth and development of plants. 1-aminocyclopropane carboxylic acid (ACC) is a precursor of ethylene synthesis, so the detection of ACC content is of great significance for the study of ethylene synthesis and plant growth regulation. In this article, we will introduce a simple, rapid, and accurate method for the detection of ACC content.
2. Materials and methods.
1.Reagents & Instruments.
The reagents required for this experiment include ACC standards, methanol, acetonitrile, phosphate buffer, etc. The instruments required for the experiment include high-performance liquid chromatograph, ultraviolet detector, centrifuge, nitrogen blowing instrument, etc.
2.Sample handling.
The plant tissue is ground into a powder, an appropriate amount of methanol is added, and it is allowed to stand in an ice bath for 10 min before centrifugation to obtain the supernatant. The supernatant nitrogen is blown to nearly dry, reconstituted with acetonitrile, and finally stabilized to an appropriate volume with phosphate buffer.
3.High performance liquid chromatography analysis.
High performance liquid chromatography was used for separation and detection. The column was a C18 column with a mixture of acetonitrile and phosphate buffer. The detection wavelength is 220nm. Qualitative analysis was performed by comparing the retention time and UV absorption spectra of standards and samples, and quantitatively using the peak area method.
III. Results and Discussion.
1.Experimental results.
By high-performance liquid chromatography analysis, we obtained data on the content of ACC in different samples. These data showed that there were significant differences in ACC content in different plant tissues. At the same time, we also found that the ACC content gradually increased with the extension of plant growth time.
2.Discuss.
In this study, a simple, rapid and accurate method for the detection of ACC content was established. This method has high sensitivity and accuracy, and is suitable for the determination of ACC content in plant tissues. Through this experiment, we can better understand the mechanism of action of plant growth regulators and provide strong support for agricultural production. At the same time, this experiment also provides a certain experimental basis for further research on ethylene synthesis precursors in the future.
IV. Conclusions. In this paper, a simple, rapid and accurate method for the detection of ACC content was established, which provided an important experimental basis for the study of ethylene synthesis and plant growth regulation. Through this experiment, we can better understand the mechanism of action of plant growth regulators and provide strong support for agricultural production. At the same time, this experiment also provides a certain experimental basis for further research on ethylene synthesis precursors in the future.