Abscisic acid and gibberellin are important hormones in plant growth and play an important role in regulating plant growth and development. In agricultural production, it is of great significance to understand the content of abscisic acid and gibberellin in plants to guide agricultural production. Therefore, the research on the separation and detection methods of abscisic acid and gibberellin has important practical application value.
1. Separation and detection of abscisic acid.
Abscisic acid is a plant hormone with a wide range of physiological activities, which plays an important role in regulating the growth and development of plants. In agricultural production, it is of great significance to understand the content of abscisic acid in plants to guide agricultural production.
1.Separation of abscisic acid.
A variety of methods can be used for the separation of abscisic acid, among which commonly used methods include solvent extraction, chromatography, membrane separation, etc. Among them, solvent extraction is one of the most commonly used methods, which mainly uses the different solubility of abscisic acid in different solvents to separate abscisic acid from plant tissues through multiple extractions. Chromatography is also a commonly used method to separate abscisic acid from other substances by using the adsorption and desorption of different substances between the stationary and mobile phases. The membrane separation method is to use the selective permeability of the membrane to separate abscisic acid from plant tissues.
2.Detection of abscisic acid.
A variety of methods can be used for the detection of abscisic acid, among which the commonly used methods include fluorescence analysis, ultraviolet-visible spectrophotometry, high performance liquid chromatography, etc. Among them, fluorescence analysis is a highly sensitive method, which mainly uses the fluorescence properties of abscisic acid at specific wavelengths for quantitative analysis. UV-Vis spectrophotometry is also a commonly used method, which mainly uses the absorbance properties of abscisic acid at specific wavelengths for quantitative analysis. High performance liquid chromatography (HPLC) is a method that separates abscisic acid from other substances and analyzes it qualitatively and quantitatively.
2. Isolation and detection of gibberellin.
Gibberellin is a plant hormone with a wide range of physiological activities, and also plays an important role in regulating the growth and development of plants. In agricultural production, understanding the content of gibberellin in plants is also of great significance to guide agricultural production.
1.Isolation of gibberellin.
Gibberellin can be isolated by a variety of methods, among which commonly used methods include solvent extraction, enzyme-linked immunosorbent assay, etc. Among them, solvent extraction is one of the most commonly used methods, which mainly uses the different solubility of gibberellin in different solvents to separate gibberellin from plant tissues through multiple extractions. Enzyme-linked immunosorbent assay is a specific isolation method that uses the specific binding between antibodies and antigens to isolate gibberellin from plant tissues.
2.Detection of gibberellin.
A variety of methods can be used for the detection of gibberellin, among which the commonly used methods include fluorescence analysis, ultraviolet-visible spectrophotometry, high performance liquid chromatography, etc. Among them, fluorescence analysis is a method with high sensitivity, which mainly uses the fluorescence properties of gibberellin at specific wavelengths for quantitative analysis. UV/VIS spectrophotometry is also a commonly used method that uses the absorbance properties of gibberellin at specific wavelengths for quantitative analysis. High performance liquid chromatography (HPLC) is a method that separates gibberellin from other substances and analyzes them qualitatively and quantitatively.
In conclusion, abscisic acid and gibberellin are important hormones in plant growth and play an important role in regulating the growth and development of plants. Understanding the levels of abscisic acid and gibberellin in plants is of great significance for guiding agricultural production. Therefore, the research on the separation and detection methods of abscisic acid and gibberellin has important practical application value.